Biodistribution and lymphatic tracking of the main neurotoxin of micrurus fulvius venom by molecular imaging

Irene Vergara, Erick Y. Castillo, Mario E. Romero-Piña, Itzel Torres-Viquez, Dayanira Paniagua, Leslie V. Boyer, Alejandro Alagón, Luis Alberto Medina

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The venom of the Eastern coral snake Micrurus fulvius can cause respiratory paralysis in the bitten patient, which is attributable to β-neurotoxins (β -NTx). The aim of this work was to study the biodistribution and lymphatic tracking by molecular imaging of the main β -NTx of M. fulvius venom. β -NTx was bioconjugated with the chelator diethylenetriaminepenta-acetic acid (DTPA) and radiolabeled with the radionuclide Gallium-67. Radiolabeling efficiency was 60%-78%; radiochemical purity ≥92%; and stability at 48 h ≥ 85%. The median lethal dose (LD50) and PLA2 activity of bioconjugated β -NTx decreased 3 and 2.5 times, respectively, in comparison with native β -NTx. The immune recognition by polyclonal antibodies decreased 10 times. Biodistribution of β -NTx-DTPA-67Ga in rats showed increased uptake in popliteal, lumbar nodes and kidneys that was not observed with67Ga-free. Accumulation in organs at 24 h was less than 1%, except for kidneys, where the average was 3.7%. The inoculation site works as a depot, since 10% of the initial dose of β -NTx-DTPA-67Ga remains there for up to 48 h. This work clearly demonstrates the lymphatic system participation in the biodistribution of β -NTx-DTPA-67Ga. Our approach could be applied to analyze the role of the lymphatic system in snakebite for a better understanding of envenoming.

Original languageEnglish (US)
Article number85
JournalToxins
Volume8
Issue number4
DOIs
StatePublished - Mar 26 2016

Keywords

  • Coral snake
  • Lymphatic absorption
  • Molecular imaging
  • Neurotoxin
  • Radiolabeling

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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    Vergara, I., Castillo, E. Y., Romero-Piña, M. E., Torres-Viquez, I., Paniagua, D., Boyer, L. V., Alagón, A., & Medina, L. A. (2016). Biodistribution and lymphatic tracking of the main neurotoxin of micrurus fulvius venom by molecular imaging. Toxins, 8(4), [85]. https://doi.org/10.3390/toxins8040085