Abstract
This paper reports the development of a biodosimetry device suitable for rapidly measuring expression levels of a low-density gene set that can define radiation exposure, dose and injury in a public health emergency. The platform comprises a set of 14 genes selected on the basis of their abundance and differential expression level in response to radiation from an expression profiling series measuring 41,000 transcripts. Gene expression is analyzed through direct signal amplification using a quantitative Nuclease Protection Assay (qNPA). This assay can be configured as either a high-throughput microplate assay or as a handheld detection device for individual point-of-care assays. Recently, we were able to successfully develop the qNPA platform to measure gene expression levels directly from human whole blood samples. The assay can be performed with volumes as small as 30 μL of whole blood, which is compatible with collection from a fingerstick. We analyzed in vitro irradiated blood samples with qNPA. The results revealed statistically significant discrimination between irradiated and non-irradiated samples. These results indicate that the qNPA platform combined with a gene profile based on a small number of genes is a valid test to measure biological radiation exposure. The scalability characteristics of the assay make it appropriate for population triage. This biodosimetry platform could also be used for personalized monitoring of radiotherapy treatments received by patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 179-185 |
| Number of pages | 7 |
| Journal | Health Physics |
| Volume | 98 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2010 |
Fingerprint
Keywords
- Accidents
- Blood
- Dosimetry
- Handling
- Radiological terrorism
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Health, Toxicology and Mutagenesis
- Epidemiology
Cite this
Biodosimetry on small blood volume using gene expression assay. / Brengues, Muriel; Paap, Brigitte; Bittner, Michael; Amundson, Sally; Seligmann, Bruce; Korn, Ronald; Lenigk, Ralf; Zenhausern, Frederic.
In: Health Physics, Vol. 98, No. 2, 02.2010, p. 179-185.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Biodosimetry on small blood volume using gene expression assay
AU - Brengues, Muriel
AU - Paap, Brigitte
AU - Bittner, Michael
AU - Amundson, Sally
AU - Seligmann, Bruce
AU - Korn, Ronald
AU - Lenigk, Ralf
AU - Zenhausern, Frederic
PY - 2010/2
Y1 - 2010/2
N2 - This paper reports the development of a biodosimetry device suitable for rapidly measuring expression levels of a low-density gene set that can define radiation exposure, dose and injury in a public health emergency. The platform comprises a set of 14 genes selected on the basis of their abundance and differential expression level in response to radiation from an expression profiling series measuring 41,000 transcripts. Gene expression is analyzed through direct signal amplification using a quantitative Nuclease Protection Assay (qNPA). This assay can be configured as either a high-throughput microplate assay or as a handheld detection device for individual point-of-care assays. Recently, we were able to successfully develop the qNPA platform to measure gene expression levels directly from human whole blood samples. The assay can be performed with volumes as small as 30 μL of whole blood, which is compatible with collection from a fingerstick. We analyzed in vitro irradiated blood samples with qNPA. The results revealed statistically significant discrimination between irradiated and non-irradiated samples. These results indicate that the qNPA platform combined with a gene profile based on a small number of genes is a valid test to measure biological radiation exposure. The scalability characteristics of the assay make it appropriate for population triage. This biodosimetry platform could also be used for personalized monitoring of radiotherapy treatments received by patients.
AB - This paper reports the development of a biodosimetry device suitable for rapidly measuring expression levels of a low-density gene set that can define radiation exposure, dose and injury in a public health emergency. The platform comprises a set of 14 genes selected on the basis of their abundance and differential expression level in response to radiation from an expression profiling series measuring 41,000 transcripts. Gene expression is analyzed through direct signal amplification using a quantitative Nuclease Protection Assay (qNPA). This assay can be configured as either a high-throughput microplate assay or as a handheld detection device for individual point-of-care assays. Recently, we were able to successfully develop the qNPA platform to measure gene expression levels directly from human whole blood samples. The assay can be performed with volumes as small as 30 μL of whole blood, which is compatible with collection from a fingerstick. We analyzed in vitro irradiated blood samples with qNPA. The results revealed statistically significant discrimination between irradiated and non-irradiated samples. These results indicate that the qNPA platform combined with a gene profile based on a small number of genes is a valid test to measure biological radiation exposure. The scalability characteristics of the assay make it appropriate for population triage. This biodosimetry platform could also be used for personalized monitoring of radiotherapy treatments received by patients.
KW - Accidents
KW - Blood
KW - Dosimetry
KW - Handling
KW - Radiological terrorism
UR - http://www.scopus.com/inward/record.url?scp=75649110801&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=75649110801&partnerID=8YFLogxK
U2 - 10.1097/01.HP.0000346706.44253.5c
DO - 10.1097/01.HP.0000346706.44253.5c
M3 - Article
C2 - 20065681
AN - SCOPUS:75649110801
VL - 98
SP - 179
EP - 185
JO - Health Physics
JF - Health Physics
SN - 0017-9078
IS - 2
ER -