Bioethanol production from the macroalgae Sargassum spp.

Myra G. Borines, Rizalinda L. de Leon, Joel L. Cuello

Research output: Contribution to journalArticlepeer-review

148 Scopus citations


Macroalgae, an abundant and carbon-neutral renewable resource, with several species rich in carbohydrates are suitable for bioethanol production. This study focused on the pretreatment, enzyme saccharification and fermentation of Sargassum spp., a brown macroalgae for bioethanol production. The optimal acid pretreatment condition achieved in terms of glucose and reducing sugar yields was 3.4-4.6% (w/v) H2SO4 concentration, 115°C and 1.50h. The pretreated biomass was hydrolyzed with cellulase enzyme system supplemented with β-glucosidase. After fermentation by Saccharomyces cerevisiae at 40°C, pH of 4.5 for 48h, the ethanol conversion rate of the enzyme hydrolysate reached 89%, which was markedly higher than the theoretical yield of 51% based on glucose as substrate. Since all the glucose was consumed during fermentation, other sugar sources might be present in the hydrolysate. The macroalgae, Sargassum spp., showed significant potential as a renewable feedstock for the production of bioethanol.

Original languageEnglish (US)
Pages (from-to)22-29
Number of pages8
JournalBioresource Technology
StatePublished - Jun 2013


  • Alternative fuel
  • Bioethanol
  • Fermentation
  • Macroalgae
  • Sargassum

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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