Biofilms correlate with TH1 inflammation in the sinonasal tissue of patients with chronic rhinosinusitis

Adrianna M. Hekiert, Jennifer M. Kofonow, Laurel Doghramji, David W. Kennedy, Alexander G Chiu, James N. Palmer, Jeffery G. Leid, Noam A. Cohen

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Objective: Determine the prevalence of bacterial biofilms in surgical chronic rhinosinusitis (CRS) patients and characterize the inflammatory response associated with biofilm CRS. Study Design: Cross-sectional. Setting: Tertiary care academic center. Subjects and Methods: Sinonasal mucosa and peripheral blood were collected from 60 CRS patients. Mucosal biofilms were demonstrated by scanning electron microscopy. Leukocyte subpopulations were determined by flow cytometry. Cytokines were identified with a luminex-based assay on the lysate of homogenized tissue or plasma. Results: Of the 60 samples, 17 were determined to be positive for the presence of biofilms. Oral steroid-naive CRS patients with biofilm demonstrated a local TH1 inflammatory response with significantly elevated levels of interferon-γ (INF-γ), granulocyte colony-stimulating factor, macrophage inflammatory protein-1 β, and neutrophils in the sinonasal mucosa. No differences were present at the systemic level. Conclusion: Sinonasal bacterial biofilms correlate to a TH1 skewed local but not systemic inflammatory response in CRS. This difference is abrogated by the use of oral steroids.

Original languageEnglish (US)
Pages (from-to)448-453
Number of pages6
JournalOtolaryngology - Head and Neck Surgery
Volume141
Issue number4
DOIs
StatePublished - Oct 2009
Externally publishedYes

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Biofilms
Inflammation
Mucous Membrane
Steroids
Macrophage Inflammatory Proteins
Granulocyte Colony-Stimulating Factor
Tertiary Care Centers
Electron Scanning Microscopy
Interferons
Flow Cytometry
Neutrophils
Leukocytes
Cross-Sectional Studies
Cytokines

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery
  • Medicine(all)

Cite this

Biofilms correlate with TH1 inflammation in the sinonasal tissue of patients with chronic rhinosinusitis. / Hekiert, Adrianna M.; Kofonow, Jennifer M.; Doghramji, Laurel; Kennedy, David W.; Chiu, Alexander G; Palmer, James N.; Leid, Jeffery G.; Cohen, Noam A.

In: Otolaryngology - Head and Neck Surgery, Vol. 141, No. 4, 10.2009, p. 448-453.

Research output: Contribution to journalArticle

Hekiert, Adrianna M. ; Kofonow, Jennifer M. ; Doghramji, Laurel ; Kennedy, David W. ; Chiu, Alexander G ; Palmer, James N. ; Leid, Jeffery G. ; Cohen, Noam A. / Biofilms correlate with TH1 inflammation in the sinonasal tissue of patients with chronic rhinosinusitis. In: Otolaryngology - Head and Neck Surgery. 2009 ; Vol. 141, No. 4. pp. 448-453.
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AU - Kofonow, Jennifer M.

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AU - Kennedy, David W.

AU - Chiu, Alexander G

AU - Palmer, James N.

AU - Leid, Jeffery G.

AU - Cohen, Noam A.

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