Biogenesis of the apical endosome-lysosome complex during differentiation of absorptive epithelial cells in rat ileum

Jean Wilson, J. Andrew Whitney, Marian R. Neutra

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Absorptive cells of the neonatal rat ileum have an elaborate apical endocytic complex consisting of tubular and vesicular endosomes, multivesicular bodies (MVB), and a giant lysosomal vacuole. This system develops rapidly over the last 3 days (20-22) of gestation. We followed the assembly of this complex by ultrastructural analysis and immunocytochemistry using antigenic markers for microvilli, endosomal tubules and lysosomal membranes. At 19 days gestation, low levels of lactase appeared on microvilli but specialized apical endosomal tubules and lysosomes were absent. At 20 days, expression of microvillar lactase increased and the endosomal marker entubin appeared, in parallel with the appearance of specialized apical endosomal tubules. The compartments of the apical endosomelysosome system were assembled sequentially after differentiation of the apical plasma membrane domains; first endosomal tubules and vesicles, followed by MVB, and ending with the assembly of the giant lysosome shortly after birth. During early stages of the assembly process, membrane components of the tubular endosomes and lysosomes appeared in the apical plasma membrane before being restricted to their respective intracellular compartments.

Original languageEnglish (US)
Pages (from-to)133-143
Number of pages11
JournalJournal of Cell Science
Volume100
Issue number1
StatePublished - Sep 1991

Fingerprint

Endosomes
Lysosomes
Ileum
Multivesicular Bodies
Lactase
Epithelial Cells
Microvilli
Cell Membrane
Pregnancy
Membranes
Vacuoles
Immunohistochemistry
Parturition

Keywords

  • Development
  • Endosomes
  • Intestine
  • Lysosomes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Biogenesis of the apical endosome-lysosome complex during differentiation of absorptive epithelial cells in rat ileum. / Wilson, Jean; Whitney, J. Andrew; Neutra, Marian R.

In: Journal of Cell Science, Vol. 100, No. 1, 09.1991, p. 133-143.

Research output: Contribution to journalArticle

@article{8194a63be23b445fb60d8f00cecfcb84,
title = "Biogenesis of the apical endosome-lysosome complex during differentiation of absorptive epithelial cells in rat ileum",
abstract = "Absorptive cells of the neonatal rat ileum have an elaborate apical endocytic complex consisting of tubular and vesicular endosomes, multivesicular bodies (MVB), and a giant lysosomal vacuole. This system develops rapidly over the last 3 days (20-22) of gestation. We followed the assembly of this complex by ultrastructural analysis and immunocytochemistry using antigenic markers for microvilli, endosomal tubules and lysosomal membranes. At 19 days gestation, low levels of lactase appeared on microvilli but specialized apical endosomal tubules and lysosomes were absent. At 20 days, expression of microvillar lactase increased and the endosomal marker entubin appeared, in parallel with the appearance of specialized apical endosomal tubules. The compartments of the apical endosomelysosome system were assembled sequentially after differentiation of the apical plasma membrane domains; first endosomal tubules and vesicles, followed by MVB, and ending with the assembly of the giant lysosome shortly after birth. During early stages of the assembly process, membrane components of the tubular endosomes and lysosomes appeared in the apical plasma membrane before being restricted to their respective intracellular compartments.",
keywords = "Development, Endosomes, Intestine, Lysosomes",
author = "Jean Wilson and Whitney, {J. Andrew} and Neutra, {Marian R.}",
year = "1991",
month = "9",
language = "English (US)",
volume = "100",
pages = "133--143",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "1",

}

TY - JOUR

T1 - Biogenesis of the apical endosome-lysosome complex during differentiation of absorptive epithelial cells in rat ileum

AU - Wilson, Jean

AU - Whitney, J. Andrew

AU - Neutra, Marian R.

PY - 1991/9

Y1 - 1991/9

N2 - Absorptive cells of the neonatal rat ileum have an elaborate apical endocytic complex consisting of tubular and vesicular endosomes, multivesicular bodies (MVB), and a giant lysosomal vacuole. This system develops rapidly over the last 3 days (20-22) of gestation. We followed the assembly of this complex by ultrastructural analysis and immunocytochemistry using antigenic markers for microvilli, endosomal tubules and lysosomal membranes. At 19 days gestation, low levels of lactase appeared on microvilli but specialized apical endosomal tubules and lysosomes were absent. At 20 days, expression of microvillar lactase increased and the endosomal marker entubin appeared, in parallel with the appearance of specialized apical endosomal tubules. The compartments of the apical endosomelysosome system were assembled sequentially after differentiation of the apical plasma membrane domains; first endosomal tubules and vesicles, followed by MVB, and ending with the assembly of the giant lysosome shortly after birth. During early stages of the assembly process, membrane components of the tubular endosomes and lysosomes appeared in the apical plasma membrane before being restricted to their respective intracellular compartments.

AB - Absorptive cells of the neonatal rat ileum have an elaborate apical endocytic complex consisting of tubular and vesicular endosomes, multivesicular bodies (MVB), and a giant lysosomal vacuole. This system develops rapidly over the last 3 days (20-22) of gestation. We followed the assembly of this complex by ultrastructural analysis and immunocytochemistry using antigenic markers for microvilli, endosomal tubules and lysosomal membranes. At 19 days gestation, low levels of lactase appeared on microvilli but specialized apical endosomal tubules and lysosomes were absent. At 20 days, expression of microvillar lactase increased and the endosomal marker entubin appeared, in parallel with the appearance of specialized apical endosomal tubules. The compartments of the apical endosomelysosome system were assembled sequentially after differentiation of the apical plasma membrane domains; first endosomal tubules and vesicles, followed by MVB, and ending with the assembly of the giant lysosome shortly after birth. During early stages of the assembly process, membrane components of the tubular endosomes and lysosomes appeared in the apical plasma membrane before being restricted to their respective intracellular compartments.

KW - Development

KW - Endosomes

KW - Intestine

KW - Lysosomes

UR - http://www.scopus.com/inward/record.url?scp=0025895405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025895405&partnerID=8YFLogxK

M3 - Article

C2 - 1795021

AN - SCOPUS:0025895405

VL - 100

SP - 133

EP - 143

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 1

ER -