Biological and anatomical evidence for kisspeptin regulation of the hypothalamic-pituitary-gonadal axis of estrous horse mares

Christianne Magee, Chad D. Foradori, Jason E. Bruemmer, Jesus A. Arreguin-Arevalo, Patrick M. McCue, Robert J Handa, Edward L. Squires, Colin M. Clay

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The purpose of the present study was to evaluate the effects of kisspeptin (KiSS) on LH and FSH secretion in the seasonally estrous mare and to examine the distribution and connectivity of GnRH and KiSS neurons in the equine preoptic area (POA) and hypothalamus. The diestrous mare has a threshold serum gonadotropin response to iv rodent KiSS decapeptide (rKP-10) administration between 1.0 and 500 μg. Administration of 500 μg and 1.0 mg rKP-10 elicited peak, mean, and area under the curve LH and FSH responses indistinguishable to that of 25 μg GnRH iv, although a single iv injection of 1.0 mg rKP-10 was insufficient to induce ovulation in the estrous mare. GnRH and KiSS-immunoreactive (ir) cells were identified in the POA and hypothalamus of the diestrous mare. In addition, KiSS-ir fibers were identified in close association with 33.7% of GnRH-ir soma, suggesting a direct action of KiSS on GnRH neurons in the mare. In conclusion, we are the first to reveal a physiological role for KiSS in the diestrous mare with direct anatomic evidence by demonstrating a threshold-like gonadotropin response to KiSS administration and characterizing KiSS and GnRH-ir in the POA and hypothalamus of the diestrous horse mare.

Original languageEnglish (US)
Pages (from-to)2813-2821
Number of pages9
JournalEndocrinology
Volume150
Issue number6
DOIs
StatePublished - Jun 2009

Fingerprint

Kisspeptins
Horses
Gonadotropin-Releasing Hormone
Preoptic Area
Hypothalamus
Gonadotropins
Neurons
Carisoprodol
Ovulation
Area Under Curve
Rodentia

ASJC Scopus subject areas

  • Endocrinology

Cite this

Magee, C., Foradori, C. D., Bruemmer, J. E., Arreguin-Arevalo, J. A., McCue, P. M., Handa, R. J., ... Clay, C. M. (2009). Biological and anatomical evidence for kisspeptin regulation of the hypothalamic-pituitary-gonadal axis of estrous horse mares. Endocrinology, 150(6), 2813-2821. https://doi.org/10.1210/en.2008-1698

Biological and anatomical evidence for kisspeptin regulation of the hypothalamic-pituitary-gonadal axis of estrous horse mares. / Magee, Christianne; Foradori, Chad D.; Bruemmer, Jason E.; Arreguin-Arevalo, Jesus A.; McCue, Patrick M.; Handa, Robert J; Squires, Edward L.; Clay, Colin M.

In: Endocrinology, Vol. 150, No. 6, 06.2009, p. 2813-2821.

Research output: Contribution to journalArticle

Magee, C, Foradori, CD, Bruemmer, JE, Arreguin-Arevalo, JA, McCue, PM, Handa, RJ, Squires, EL & Clay, CM 2009, 'Biological and anatomical evidence for kisspeptin regulation of the hypothalamic-pituitary-gonadal axis of estrous horse mares', Endocrinology, vol. 150, no. 6, pp. 2813-2821. https://doi.org/10.1210/en.2008-1698
Magee, Christianne ; Foradori, Chad D. ; Bruemmer, Jason E. ; Arreguin-Arevalo, Jesus A. ; McCue, Patrick M. ; Handa, Robert J ; Squires, Edward L. ; Clay, Colin M. / Biological and anatomical evidence for kisspeptin regulation of the hypothalamic-pituitary-gonadal axis of estrous horse mares. In: Endocrinology. 2009 ; Vol. 150, No. 6. pp. 2813-2821.
@article{b0253a3e984e4cd682299fd0a6042989,
title = "Biological and anatomical evidence for kisspeptin regulation of the hypothalamic-pituitary-gonadal axis of estrous horse mares",
abstract = "The purpose of the present study was to evaluate the effects of kisspeptin (KiSS) on LH and FSH secretion in the seasonally estrous mare and to examine the distribution and connectivity of GnRH and KiSS neurons in the equine preoptic area (POA) and hypothalamus. The diestrous mare has a threshold serum gonadotropin response to iv rodent KiSS decapeptide (rKP-10) administration between 1.0 and 500 μg. Administration of 500 μg and 1.0 mg rKP-10 elicited peak, mean, and area under the curve LH and FSH responses indistinguishable to that of 25 μg GnRH iv, although a single iv injection of 1.0 mg rKP-10 was insufficient to induce ovulation in the estrous mare. GnRH and KiSS-immunoreactive (ir) cells were identified in the POA and hypothalamus of the diestrous mare. In addition, KiSS-ir fibers were identified in close association with 33.7{\%} of GnRH-ir soma, suggesting a direct action of KiSS on GnRH neurons in the mare. In conclusion, we are the first to reveal a physiological role for KiSS in the diestrous mare with direct anatomic evidence by demonstrating a threshold-like gonadotropin response to KiSS administration and characterizing KiSS and GnRH-ir in the POA and hypothalamus of the diestrous horse mare.",
author = "Christianne Magee and Foradori, {Chad D.} and Bruemmer, {Jason E.} and Arreguin-Arevalo, {Jesus A.} and McCue, {Patrick M.} and Handa, {Robert J} and Squires, {Edward L.} and Clay, {Colin M.}",
year = "2009",
month = "6",
doi = "10.1210/en.2008-1698",
language = "English (US)",
volume = "150",
pages = "2813--2821",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "6",

}

TY - JOUR

T1 - Biological and anatomical evidence for kisspeptin regulation of the hypothalamic-pituitary-gonadal axis of estrous horse mares

AU - Magee, Christianne

AU - Foradori, Chad D.

AU - Bruemmer, Jason E.

AU - Arreguin-Arevalo, Jesus A.

AU - McCue, Patrick M.

AU - Handa, Robert J

AU - Squires, Edward L.

AU - Clay, Colin M.

PY - 2009/6

Y1 - 2009/6

N2 - The purpose of the present study was to evaluate the effects of kisspeptin (KiSS) on LH and FSH secretion in the seasonally estrous mare and to examine the distribution and connectivity of GnRH and KiSS neurons in the equine preoptic area (POA) and hypothalamus. The diestrous mare has a threshold serum gonadotropin response to iv rodent KiSS decapeptide (rKP-10) administration between 1.0 and 500 μg. Administration of 500 μg and 1.0 mg rKP-10 elicited peak, mean, and area under the curve LH and FSH responses indistinguishable to that of 25 μg GnRH iv, although a single iv injection of 1.0 mg rKP-10 was insufficient to induce ovulation in the estrous mare. GnRH and KiSS-immunoreactive (ir) cells were identified in the POA and hypothalamus of the diestrous mare. In addition, KiSS-ir fibers were identified in close association with 33.7% of GnRH-ir soma, suggesting a direct action of KiSS on GnRH neurons in the mare. In conclusion, we are the first to reveal a physiological role for KiSS in the diestrous mare with direct anatomic evidence by demonstrating a threshold-like gonadotropin response to KiSS administration and characterizing KiSS and GnRH-ir in the POA and hypothalamus of the diestrous horse mare.

AB - The purpose of the present study was to evaluate the effects of kisspeptin (KiSS) on LH and FSH secretion in the seasonally estrous mare and to examine the distribution and connectivity of GnRH and KiSS neurons in the equine preoptic area (POA) and hypothalamus. The diestrous mare has a threshold serum gonadotropin response to iv rodent KiSS decapeptide (rKP-10) administration between 1.0 and 500 μg. Administration of 500 μg and 1.0 mg rKP-10 elicited peak, mean, and area under the curve LH and FSH responses indistinguishable to that of 25 μg GnRH iv, although a single iv injection of 1.0 mg rKP-10 was insufficient to induce ovulation in the estrous mare. GnRH and KiSS-immunoreactive (ir) cells were identified in the POA and hypothalamus of the diestrous mare. In addition, KiSS-ir fibers were identified in close association with 33.7% of GnRH-ir soma, suggesting a direct action of KiSS on GnRH neurons in the mare. In conclusion, we are the first to reveal a physiological role for KiSS in the diestrous mare with direct anatomic evidence by demonstrating a threshold-like gonadotropin response to KiSS administration and characterizing KiSS and GnRH-ir in the POA and hypothalamus of the diestrous horse mare.

UR - http://www.scopus.com/inward/record.url?scp=66649087423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66649087423&partnerID=8YFLogxK

U2 - 10.1210/en.2008-1698

DO - 10.1210/en.2008-1698

M3 - Article

C2 - 19228887

AN - SCOPUS:66649087423

VL - 150

SP - 2813

EP - 2821

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 6

ER -