Biological properties and cell tropism of Chp2, a bacteriophage of the obligate intracellular bacterium Chlamydophila abortus

J. S. Everson, S. A. Garner, Bentley A Fane, B. L. Liu, P. R. Lambden, I. N. Clarke

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A number of bacteriophages belonging to the Microviridae have been described infecting chlamydiae. Phylogenetic studies divide the Chlamydiaceae into two distinct genera, Chlamydia and Chlamydophila, containing three and six different species, respectively. In this work we investigated the biological properties and host range of the recently described bacteriophage Chp2 that was originally discovered in Chlamydophila abortus. The obligate intracellular development cycle of chlamydiae has precluded the development of quantitative approaches to assay bacteriophage infectivity. Thus, we prepared hybridomas secreting monoclonal antibodies (monoclonal antibodies 40 and 55) that were specific for Chp2. We demonstrated that Chp2 binds both C. abortus elementary bodies and reticulate bodies in an enzyme-linked immunosorbent assay. Monoclonal antibodies 40 and 55 also detected bacteriophage Chp2 antigens in chlamydia-infected eukaryotic cells. We used these monoclonal antibodies to monitor the ability of Chp2 to infect all nine species of chlamydiae. Chp2 does not infect members of the genus Chlamydia (C. trachomatis, C. suis, or C. muridarum). Chp2 can infect C. abortus, C. felis, and C. pecorum but is unable to infect other members of this genus, including C. caviae and C. pneumoniae, despite the fact that these chlamydial species support the replication of very closely related bacteriophages.

Original languageEnglish (US)
Pages (from-to)2748-2754
Number of pages7
JournalJournal of Bacteriology
Volume184
Issue number10
DOIs
StatePublished - 2002
Externally publishedYes

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Chlamydophila
Tropism
Chlamydia
Bacteriophages
Bacteria
Monoclonal Antibodies
Microviridae
Chlamydiaceae
Felis
Host Specificity
Chlamydia trachomatis
Hybridomas
Eukaryotic Cells
Pneumonia
Enzyme-Linked Immunosorbent Assay
Antigens

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Biological properties and cell tropism of Chp2, a bacteriophage of the obligate intracellular bacterium Chlamydophila abortus. / Everson, J. S.; Garner, S. A.; Fane, Bentley A; Liu, B. L.; Lambden, P. R.; Clarke, I. N.

In: Journal of Bacteriology, Vol. 184, No. 10, 2002, p. 2748-2754.

Research output: Contribution to journalArticle

Everson, J. S. ; Garner, S. A. ; Fane, Bentley A ; Liu, B. L. ; Lambden, P. R. ; Clarke, I. N. / Biological properties and cell tropism of Chp2, a bacteriophage of the obligate intracellular bacterium Chlamydophila abortus. In: Journal of Bacteriology. 2002 ; Vol. 184, No. 10. pp. 2748-2754.
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