Biomolecular interactions control the shape of stains from drying droplets of complex fluids

Cedric Hurth, Rajneesh Bhardwaj, Sahar Andalib, Christophe Frankiewicz, Andrew Dobos, Daniel Attinger, Frederic Zenhausern

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

When a sessile droplet of a complex fluid dries, a stain forms on the solid surface. The structure and pattern of the stain can be used to detect the presence of a specific chemical compound in the sessile droplet. In the present work, we investigate what parameters of the stain or its formation can be used to characterize the specific interaction between an aqueous dispersion of beads and its receptor immobilized on the surface. We use the biotin-streptavidin system as an experimental model. Clear dissimilarities were observed in the drying sequences on streptavidin-coated substrates of droplets of aqueous solutions containing biotin-coated or streptavidin-coated beads. Fluorescent beads are used in order to visualize the fluid flow field. We show differences in the distribution of the particles on the surface depending on biomolecular interactions between beads and the solid surface. A mechanistic model is proposed to explain the different patterns obtained during drying. The model describes that the beads are left behind the receding wetting line rather than pulled towards the drop center if the biological binding force is comparable to the surface tension of the receding wetting line. Other forces such as the viscous drag, van der Waals forces, and solid-solid friction forces are found negligible. Simple microfluidics experiments are performed to further illustrate the difference in behavior where is adhesion or friction are present between the bead and substrate due to the biological force. The results of the model are in agreement with the experimental observations which provide insight and design capabilities. A better understanding of the effects of the droplet-surface interaction on the drying mechanism is a crucial first step before the identification of drying patterns can be promisingly applied to areas such as immunology and biomarker detection.

Original languageEnglish (US)
Pages (from-to)398-403
Number of pages6
JournalChemical Engineering Science
Volume137
DOIs
StatePublished - Dec 1 2015

Fingerprint

Complex Fluids
Drying
Coloring Agents
Streptavidin
Fluids
Interaction
Wetting
Biotin
Droplet
Friction
Substrate
Immunology
Van Der Waals Force
Van der Waals forces
Chemical compounds
Line
Microfluidics
Biomarkers
Dissimilarity
Substrates

Keywords

  • Biosensors
  • Biotin-streptavidin
  • Drying mechanism
  • Microdroplets
  • Polystyrene beads

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)
  • Applied Mathematics
  • Industrial and Manufacturing Engineering

Cite this

Biomolecular interactions control the shape of stains from drying droplets of complex fluids. / Hurth, Cedric; Bhardwaj, Rajneesh; Andalib, Sahar; Frankiewicz, Christophe; Dobos, Andrew; Attinger, Daniel; Zenhausern, Frederic.

In: Chemical Engineering Science, Vol. 137, 01.12.2015, p. 398-403.

Research output: Contribution to journalArticle

Hurth, Cedric ; Bhardwaj, Rajneesh ; Andalib, Sahar ; Frankiewicz, Christophe ; Dobos, Andrew ; Attinger, Daniel ; Zenhausern, Frederic. / Biomolecular interactions control the shape of stains from drying droplets of complex fluids. In: Chemical Engineering Science. 2015 ; Vol. 137. pp. 398-403.
@article{699b0d9deb854c4fa2d50fb534feaffa,
title = "Biomolecular interactions control the shape of stains from drying droplets of complex fluids",
abstract = "When a sessile droplet of a complex fluid dries, a stain forms on the solid surface. The structure and pattern of the stain can be used to detect the presence of a specific chemical compound in the sessile droplet. In the present work, we investigate what parameters of the stain or its formation can be used to characterize the specific interaction between an aqueous dispersion of beads and its receptor immobilized on the surface. We use the biotin-streptavidin system as an experimental model. Clear dissimilarities were observed in the drying sequences on streptavidin-coated substrates of droplets of aqueous solutions containing biotin-coated or streptavidin-coated beads. Fluorescent beads are used in order to visualize the fluid flow field. We show differences in the distribution of the particles on the surface depending on biomolecular interactions between beads and the solid surface. A mechanistic model is proposed to explain the different patterns obtained during drying. The model describes that the beads are left behind the receding wetting line rather than pulled towards the drop center if the biological binding force is comparable to the surface tension of the receding wetting line. Other forces such as the viscous drag, van der Waals forces, and solid-solid friction forces are found negligible. Simple microfluidics experiments are performed to further illustrate the difference in behavior where is adhesion or friction are present between the bead and substrate due to the biological force. The results of the model are in agreement with the experimental observations which provide insight and design capabilities. A better understanding of the effects of the droplet-surface interaction on the drying mechanism is a crucial first step before the identification of drying patterns can be promisingly applied to areas such as immunology and biomarker detection.",
keywords = "Biosensors, Biotin-streptavidin, Drying mechanism, Microdroplets, Polystyrene beads",
author = "Cedric Hurth and Rajneesh Bhardwaj and Sahar Andalib and Christophe Frankiewicz and Andrew Dobos and Daniel Attinger and Frederic Zenhausern",
year = "2015",
month = "12",
day = "1",
doi = "10.1016/j.ces.2015.06.059",
language = "English (US)",
volume = "137",
pages = "398--403",
journal = "Chemical Engineering Science",
issn = "0009-2509",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Biomolecular interactions control the shape of stains from drying droplets of complex fluids

AU - Hurth, Cedric

AU - Bhardwaj, Rajneesh

AU - Andalib, Sahar

AU - Frankiewicz, Christophe

AU - Dobos, Andrew

AU - Attinger, Daniel

AU - Zenhausern, Frederic

PY - 2015/12/1

Y1 - 2015/12/1

N2 - When a sessile droplet of a complex fluid dries, a stain forms on the solid surface. The structure and pattern of the stain can be used to detect the presence of a specific chemical compound in the sessile droplet. In the present work, we investigate what parameters of the stain or its formation can be used to characterize the specific interaction between an aqueous dispersion of beads and its receptor immobilized on the surface. We use the biotin-streptavidin system as an experimental model. Clear dissimilarities were observed in the drying sequences on streptavidin-coated substrates of droplets of aqueous solutions containing biotin-coated or streptavidin-coated beads. Fluorescent beads are used in order to visualize the fluid flow field. We show differences in the distribution of the particles on the surface depending on biomolecular interactions between beads and the solid surface. A mechanistic model is proposed to explain the different patterns obtained during drying. The model describes that the beads are left behind the receding wetting line rather than pulled towards the drop center if the biological binding force is comparable to the surface tension of the receding wetting line. Other forces such as the viscous drag, van der Waals forces, and solid-solid friction forces are found negligible. Simple microfluidics experiments are performed to further illustrate the difference in behavior where is adhesion or friction are present between the bead and substrate due to the biological force. The results of the model are in agreement with the experimental observations which provide insight and design capabilities. A better understanding of the effects of the droplet-surface interaction on the drying mechanism is a crucial first step before the identification of drying patterns can be promisingly applied to areas such as immunology and biomarker detection.

AB - When a sessile droplet of a complex fluid dries, a stain forms on the solid surface. The structure and pattern of the stain can be used to detect the presence of a specific chemical compound in the sessile droplet. In the present work, we investigate what parameters of the stain or its formation can be used to characterize the specific interaction between an aqueous dispersion of beads and its receptor immobilized on the surface. We use the biotin-streptavidin system as an experimental model. Clear dissimilarities were observed in the drying sequences on streptavidin-coated substrates of droplets of aqueous solutions containing biotin-coated or streptavidin-coated beads. Fluorescent beads are used in order to visualize the fluid flow field. We show differences in the distribution of the particles on the surface depending on biomolecular interactions between beads and the solid surface. A mechanistic model is proposed to explain the different patterns obtained during drying. The model describes that the beads are left behind the receding wetting line rather than pulled towards the drop center if the biological binding force is comparable to the surface tension of the receding wetting line. Other forces such as the viscous drag, van der Waals forces, and solid-solid friction forces are found negligible. Simple microfluidics experiments are performed to further illustrate the difference in behavior where is adhesion or friction are present between the bead and substrate due to the biological force. The results of the model are in agreement with the experimental observations which provide insight and design capabilities. A better understanding of the effects of the droplet-surface interaction on the drying mechanism is a crucial first step before the identification of drying patterns can be promisingly applied to areas such as immunology and biomarker detection.

KW - Biosensors

KW - Biotin-streptavidin

KW - Drying mechanism

KW - Microdroplets

KW - Polystyrene beads

UR - http://www.scopus.com/inward/record.url?scp=84937203175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84937203175&partnerID=8YFLogxK

U2 - 10.1016/j.ces.2015.06.059

DO - 10.1016/j.ces.2015.06.059

M3 - Article

AN - SCOPUS:84937203175

VL - 137

SP - 398

EP - 403

JO - Chemical Engineering Science

JF - Chemical Engineering Science

SN - 0009-2509

ER -