Biosynthesis and secretion of follicle-stimulating hormone β-subunit from ovine pituitary cultures: Effect of 17β-estradiol treatment

G. Kerr Whitfield, William L. Miller

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

An assay was developed to detect tritium-labeled ovine FSH β-subunit ([3H]oFSHβ) secreted from primary ovine pituitary cultures. This procedure used affinity-enriched antibodies raised against reduced and carbamylmethylated oFSHβ (RCM-oFSHβ) in a two-cycle immunoextraction procedure. A discrete species with an apparent rhol wt of 21,000 was detected in sodium dodecyl sulfate electrophoretic patterns of immunoextracts from culture medium. This species was identified as RCM- [3H]oFSHβ by its comigration with highly purified RCM oFSHβ, its reduction in culture media after cultures were treated with 17β-estradiol, which normally decreases radioimmunoassayable oFSH; and its displacement from the extracting antibodies by excess unlabeled RCM-oFSHβ. The assay was used in a pulse-chase study to determine that [3H]oFSHβ is secreted within 1-2 h of its synthesis. Prior treatment of cultures with 17β-estradiol did not change this timing of secretion.

Original languageEnglish (US)
Pages (from-to)154-159
Number of pages6
JournalEndocrinology
Volume115
Issue number1
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • Endocrinology

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