Biosynthesis of the antifungal polyketide antibiotic soraphen A in Sorangium cellulosum and Streptomyces lividans

Istvan Molnar, Ross Zirkle, James M. Ligón

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The derivatization of soraphen by combinatorial biosynthesis has been a longstanding goal of the Natural Product Genetics group at Ciba Geigy - Novartis - Syngenta. We have cloned and characterized the soraphen A biosynthetic gene cluster of Sorangium cellulosum So ce26. To facilitate the genetic manipulation of So ce26, a central regulator of gliding motility was identified and its encoding gene disrupted to create a nonswarming strain. Despite this improvement, engineering of soraphen biosynthesis remained elusive in So ce26. Thus, the genes for the soraphen polyketide synthase, the post-polyketide tailoring steps, and methoxymalonate biosynthesis were introduced into Streptomyces lividans. Production of soraphen A in this genetically tractable strain also required provision of benzoyl-CoA by feeding benzoate or cinnamate.

Original languageEnglish (US)
Title of host publicationACS Symposium Series
Pages217-230
Number of pages14
Volume955
StatePublished - 2007

Publication series

NameACS Symposium Series
Volume955
ISSN (Print)00976156

Fingerprint

Polyketides
Biosynthesis
Anti-Bacterial Agents
Genes
Cinnamates
Polyketide Synthases
Gene encoding
Benzoates
Biological Products
soraphen A

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Molnar, I., Zirkle, R., & Ligón, J. M. (2007). Biosynthesis of the antifungal polyketide antibiotic soraphen A in Sorangium cellulosum and Streptomyces lividans. In ACS Symposium Series (Vol. 955, pp. 217-230). (ACS Symposium Series; Vol. 955).

Biosynthesis of the antifungal polyketide antibiotic soraphen A in Sorangium cellulosum and Streptomyces lividans. / Molnar, Istvan; Zirkle, Ross; Ligón, James M.

ACS Symposium Series. Vol. 955 2007. p. 217-230 (ACS Symposium Series; Vol. 955).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Molnar, I, Zirkle, R & Ligón, JM 2007, Biosynthesis of the antifungal polyketide antibiotic soraphen A in Sorangium cellulosum and Streptomyces lividans. in ACS Symposium Series. vol. 955, ACS Symposium Series, vol. 955, pp. 217-230.
Molnar I, Zirkle R, Ligón JM. Biosynthesis of the antifungal polyketide antibiotic soraphen A in Sorangium cellulosum and Streptomyces lividans. In ACS Symposium Series. Vol. 955. 2007. p. 217-230. (ACS Symposium Series).
Molnar, Istvan ; Zirkle, Ross ; Ligón, James M. / Biosynthesis of the antifungal polyketide antibiotic soraphen A in Sorangium cellulosum and Streptomyces lividans. ACS Symposium Series. Vol. 955 2007. pp. 217-230 (ACS Symposium Series).
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