Biosynthetic pathways of brassinolide in Arabidopsis

T. Noguchi, S. Fujioka, S. Choe, S. Takatsuto, Frans Tax, S. Yoshida, Kenneth A Feldmann

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading to brassinolide (BL) in Arabidopsis. However, to date the in vivo operation of these pathways has not been fully confirmed in this species. This paper describes metabolic studies using deuterium-labeled BRs in wild-type and BR-insensitive mutant (bri1) seedlings to establish the intermediates of the biosynthetic pathway of BL in Arabidopsis. The first evidence for the conversion of campestanol to 6-deoxocathasterone and the conversion of 6-deoxocathasterone to 6-deoxoteasterone is provided. The later biosynthetic steps (6-deoxoteasterone → 3-dehydro-6-deoxoteasterone → 6-deoxotyphasterol → 6-deoxocastasterone → 6α-hydroxycastasterone → castasterone → BL) were demonstrated by stepwise metabolic experiments. Therefore, these studies complete the documentation of the late C6-oxidation pathway. The biosynthetic sequence involved in the early C6-oxidation pathway (teasterone → 3-dehydroteasterone → typhasterol → castasterone → BL) was also demonstrated. These results show that both the early and late C6-oxidation pathways are functional in Arabidopsis. In addition we report two new observations: the presence of a new branch in the pathway, C6 oxidation of 6-deoxotyphasterol to typhasterol, and increased metabolic flow in BR-insensitive mutants.

Original languageEnglish (US)
Pages (from-to)201-209
Number of pages9
JournalPlant Physiology
Volume124
Issue number1
StatePublished - 2000
Externally publishedYes

Fingerprint

Brassinosteroids
brassinolide
Biosynthetic Pathways
Arabidopsis
biochemical pathways
brassinosteroids
oxidation
Deuterium
Seedlings
Documentation
mutants
metabolic studies
deuterium
seedlings

ASJC Scopus subject areas

  • Plant Science

Cite this

Noguchi, T., Fujioka, S., Choe, S., Takatsuto, S., Tax, F., Yoshida, S., & Feldmann, K. A. (2000). Biosynthetic pathways of brassinolide in Arabidopsis. Plant Physiology, 124(1), 201-209.

Biosynthetic pathways of brassinolide in Arabidopsis. / Noguchi, T.; Fujioka, S.; Choe, S.; Takatsuto, S.; Tax, Frans; Yoshida, S.; Feldmann, Kenneth A.

In: Plant Physiology, Vol. 124, No. 1, 2000, p. 201-209.

Research output: Contribution to journalArticle

Noguchi, T, Fujioka, S, Choe, S, Takatsuto, S, Tax, F, Yoshida, S & Feldmann, KA 2000, 'Biosynthetic pathways of brassinolide in Arabidopsis', Plant Physiology, vol. 124, no. 1, pp. 201-209.
Noguchi T, Fujioka S, Choe S, Takatsuto S, Tax F, Yoshida S et al. Biosynthetic pathways of brassinolide in Arabidopsis. Plant Physiology. 2000;124(1):201-209.
Noguchi, T. ; Fujioka, S. ; Choe, S. ; Takatsuto, S. ; Tax, Frans ; Yoshida, S. ; Feldmann, Kenneth A. / Biosynthetic pathways of brassinolide in Arabidopsis. In: Plant Physiology. 2000 ; Vol. 124, No. 1. pp. 201-209.
@article{48abac7c47f04e60b667de66c33a08db,
title = "Biosynthetic pathways of brassinolide in Arabidopsis",
abstract = "Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading to brassinolide (BL) in Arabidopsis. However, to date the in vivo operation of these pathways has not been fully confirmed in this species. This paper describes metabolic studies using deuterium-labeled BRs in wild-type and BR-insensitive mutant (bri1) seedlings to establish the intermediates of the biosynthetic pathway of BL in Arabidopsis. The first evidence for the conversion of campestanol to 6-deoxocathasterone and the conversion of 6-deoxocathasterone to 6-deoxoteasterone is provided. The later biosynthetic steps (6-deoxoteasterone → 3-dehydro-6-deoxoteasterone → 6-deoxotyphasterol → 6-deoxocastasterone → 6α-hydroxycastasterone → castasterone → BL) were demonstrated by stepwise metabolic experiments. Therefore, these studies complete the documentation of the late C6-oxidation pathway. The biosynthetic sequence involved in the early C6-oxidation pathway (teasterone → 3-dehydroteasterone → typhasterol → castasterone → BL) was also demonstrated. These results show that both the early and late C6-oxidation pathways are functional in Arabidopsis. In addition we report two new observations: the presence of a new branch in the pathway, C6 oxidation of 6-deoxotyphasterol to typhasterol, and increased metabolic flow in BR-insensitive mutants.",
author = "T. Noguchi and S. Fujioka and S. Choe and S. Takatsuto and Frans Tax and S. Yoshida and Feldmann, {Kenneth A}",
year = "2000",
language = "English (US)",
volume = "124",
pages = "201--209",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "1",

}

TY - JOUR

T1 - Biosynthetic pathways of brassinolide in Arabidopsis

AU - Noguchi, T.

AU - Fujioka, S.

AU - Choe, S.

AU - Takatsuto, S.

AU - Tax, Frans

AU - Yoshida, S.

AU - Feldmann, Kenneth A

PY - 2000

Y1 - 2000

N2 - Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading to brassinolide (BL) in Arabidopsis. However, to date the in vivo operation of these pathways has not been fully confirmed in this species. This paper describes metabolic studies using deuterium-labeled BRs in wild-type and BR-insensitive mutant (bri1) seedlings to establish the intermediates of the biosynthetic pathway of BL in Arabidopsis. The first evidence for the conversion of campestanol to 6-deoxocathasterone and the conversion of 6-deoxocathasterone to 6-deoxoteasterone is provided. The later biosynthetic steps (6-deoxoteasterone → 3-dehydro-6-deoxoteasterone → 6-deoxotyphasterol → 6-deoxocastasterone → 6α-hydroxycastasterone → castasterone → BL) were demonstrated by stepwise metabolic experiments. Therefore, these studies complete the documentation of the late C6-oxidation pathway. The biosynthetic sequence involved in the early C6-oxidation pathway (teasterone → 3-dehydroteasterone → typhasterol → castasterone → BL) was also demonstrated. These results show that both the early and late C6-oxidation pathways are functional in Arabidopsis. In addition we report two new observations: the presence of a new branch in the pathway, C6 oxidation of 6-deoxotyphasterol to typhasterol, and increased metabolic flow in BR-insensitive mutants.

AB - Our previous studies on the endogenous brassinosteroids (BRs) in Arabidopsis have provided suggestive evidence for the operation of the early C6-oxidation and the late C6-oxidation pathways, leading to brassinolide (BL) in Arabidopsis. However, to date the in vivo operation of these pathways has not been fully confirmed in this species. This paper describes metabolic studies using deuterium-labeled BRs in wild-type and BR-insensitive mutant (bri1) seedlings to establish the intermediates of the biosynthetic pathway of BL in Arabidopsis. The first evidence for the conversion of campestanol to 6-deoxocathasterone and the conversion of 6-deoxocathasterone to 6-deoxoteasterone is provided. The later biosynthetic steps (6-deoxoteasterone → 3-dehydro-6-deoxoteasterone → 6-deoxotyphasterol → 6-deoxocastasterone → 6α-hydroxycastasterone → castasterone → BL) were demonstrated by stepwise metabolic experiments. Therefore, these studies complete the documentation of the late C6-oxidation pathway. The biosynthetic sequence involved in the early C6-oxidation pathway (teasterone → 3-dehydroteasterone → typhasterol → castasterone → BL) was also demonstrated. These results show that both the early and late C6-oxidation pathways are functional in Arabidopsis. In addition we report two new observations: the presence of a new branch in the pathway, C6 oxidation of 6-deoxotyphasterol to typhasterol, and increased metabolic flow in BR-insensitive mutants.

UR - http://www.scopus.com/inward/record.url?scp=0033833643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033833643&partnerID=8YFLogxK

M3 - Article

C2 - 10982435

AN - SCOPUS:0033833643

VL - 124

SP - 201

EP - 209

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 1

ER -