Biotinylated gene therapy vectors

Michael A. Barry, Samuel K. Campos, Debadyuti Ghosh, Kristen E. Adams, Hoyin Mok, George T. Mercier, M. Brandon Parrott

Research output: Contribution to journalReview articlepeer-review

19 Scopus citations

Abstract

The avidin-biotin system is a fundamental technology in biomedicine for immunolocalisation, imaging, nucleic acid blotting and protein labelling. This technology has recently been adapted for use in gene therapy vector applications to add proteins or cell-targeting ligands to non-viral and viral vectors. Two biotinylation technologies are being used in these applications: chemical biotinylation and metabolic biotinylation. In chemical biotinylation, reactive alkylating agents couple biotin to proteins by random covalent attachment to amino acid side chains. In metabolic biotinylation, proteins are genetically engineered with a biotin acceptor peptide (BAP), such that they are covalently biotinylated by cellular biotin ligases during viral vector production. Both technologies show promise for cell-targeting in vitro and in vivo, and for ligand screening applications. Metabolic biotinylation has the added feature of allowing viruses, vectors and vaccines to be produced from cells already biotinylated, thereby allowing them to purified by affinity chromatography on monomeric avidin columns.

Original languageEnglish (US)
Pages (from-to)925-940
Number of pages16
JournalExpert Opinion on Biological Therapy
Volume3
Issue number6
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

Keywords

  • Avidin
  • Biotin
  • Cell-targeting
  • Chemical biotinylation
  • Metabolic biotinylation
  • Streptavidin
  • Vector purification
  • Vector-targeting
  • Virus purification

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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