Biotinylated gene therapy vectors

Michael A. Barry, Samuel K Campos, Debadyuti Ghosh, Kristen E. Adams, Hoyin Mok, George T. Mercier, M. Brandon Parrott

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The avidin-biotin system is a fundamental technology in biomedicine for immunolocalisation, imaging, nucleic acid blotting and protein labelling. This technology has recently been adapted for use in gene therapy vector applications to add proteins or cell-targeting ligands to non-viral and viral vectors. Two biotinylation technologies are being used in these applications: chemical biotinylation and metabolic biotinylation. In chemical biotinylation, reactive alkylating agents couple biotin to proteins by random covalent attachment to amino acid side chains. In metabolic biotinylation, proteins are genetically engineered with a biotin acceptor peptide (BAP), such that they are covalently biotinylated by cellular biotin ligases during viral vector production. Both technologies show promise for cell-targeting in vitro and in vivo, and for ligand screening applications. Metabolic biotinylation has the added feature of allowing viruses, vectors and vaccines to be produced from cells already biotinylated, thereby allowing them to purified by affinity chromatography on monomeric avidin columns.

Original languageEnglish (US)
Pages (from-to)925-940
Number of pages16
JournalExpert Opinion on Biological Therapy
Volume3
Issue number6
DOIs
StatePublished - Sep 2003
Externally publishedYes

Fingerprint

Biotinylation
Gene therapy
Biotin
Genetic Therapy
Avidin
Technology
Proteins
Ligands
Affinity chromatography
Alkylating Agents
Ligases
Viruses
Labeling
Nucleic Acids
Screening
Vaccines
Affinity Chromatography
Imaging techniques
Amino Acids
Peptides

Keywords

  • Avidin
  • Biotin
  • Cell-targeting
  • Chemical biotinylation
  • Metabolic biotinylation
  • Streptavidin
  • Vector purification
  • Vector-targeting
  • Virus purification

ASJC Scopus subject areas

  • Pharmacology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics
  • Immunology

Cite this

Barry, M. A., Campos, S. K., Ghosh, D., Adams, K. E., Mok, H., Mercier, G. T., & Parrott, M. B. (2003). Biotinylated gene therapy vectors. Expert Opinion on Biological Therapy, 3(6), 925-940. https://doi.org/10.1517/14712598.3.6.925

Biotinylated gene therapy vectors. / Barry, Michael A.; Campos, Samuel K; Ghosh, Debadyuti; Adams, Kristen E.; Mok, Hoyin; Mercier, George T.; Parrott, M. Brandon.

In: Expert Opinion on Biological Therapy, Vol. 3, No. 6, 09.2003, p. 925-940.

Research output: Contribution to journalArticle

Barry, MA, Campos, SK, Ghosh, D, Adams, KE, Mok, H, Mercier, GT & Parrott, MB 2003, 'Biotinylated gene therapy vectors', Expert Opinion on Biological Therapy, vol. 3, no. 6, pp. 925-940. https://doi.org/10.1517/14712598.3.6.925
Barry MA, Campos SK, Ghosh D, Adams KE, Mok H, Mercier GT et al. Biotinylated gene therapy vectors. Expert Opinion on Biological Therapy. 2003 Sep;3(6):925-940. https://doi.org/10.1517/14712598.3.6.925
Barry, Michael A. ; Campos, Samuel K ; Ghosh, Debadyuti ; Adams, Kristen E. ; Mok, Hoyin ; Mercier, George T. ; Parrott, M. Brandon. / Biotinylated gene therapy vectors. In: Expert Opinion on Biological Therapy. 2003 ; Vol. 3, No. 6. pp. 925-940.
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