Biotransformation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by a prospective consortium and its most effective isolate Serratia marcescens

Douglas M. Young, Pat J. Unkefer, Kimberly L Ogden

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

The biotransformation of hexahydro-1,3,5-trinitro-1,3,5 triazine (RDX) has been observed in liquid culture by a consortium of bacteria found in horse manure. the consortium and were isolated. The most effective of these isolates at transforming RDX was Serratia marcescens. The biotransformation of RDX by all of these bacteria was found to occur only in the anoxic stationary phase. The process of bacterial growth and RDX biotransformation was quantified for the purpose of developing a predictive type model. Cell growth was assumed to follow Monod kinetics. All of the aerobic and anoxic growth parameters were determined: μmax, Ks, and Yx/s. RDX was found to competitively inhibit cell growth in both atmospheres. Degradation of RDX by Serratia marcescens was found to proceed through the stepwise reduction of the three nitro groups to nitroso groups. Each of these reductions was found to be first order in both component and cell concentrations. The degradation rate constant for the first step in this reduction process by the consortium was 0.022 L/g cells · h compared to 0.033 L/g cells · h for the most efficient isolate.

Original languageEnglish (US)
Pages (from-to)515-522
Number of pages8
JournalBiotechnology and Bioengineering
Volume53
Issue number5
DOIs
StatePublished - Mar 5 1997

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Serratia marcescens
Biotransformation
Cell growth
Bacteria
Degradation
Manures
Growth
Rate constants
Bacterial Physiological Phenomena
Kinetics
Liquids
Manure
Cellular Structures
cyclonite
Atmosphere
Horses

Keywords

  • biotransformation
  • RDX (hexahydro-1,3,5-trinitro-1,3,5-triazine
  • Serratia marcescens

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Biotransformation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) by a prospective consortium and its most effective isolate Serratia marcescens. / Young, Douglas M.; Unkefer, Pat J.; Ogden, Kimberly L.

In: Biotechnology and Bioengineering, Vol. 53, No. 5, 05.03.1997, p. 515-522.

Research output: Contribution to journalArticle

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