Biparental inheritance of chloroplast DNA and the existence of heteroplasmic cells in alfalfa

D. J. Lee, T. K. Blake, S. E. Smith

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Mapping of chloroplast DNA (ctDNA) restriction fragment patterns from a chlorophyll deficient mutant and two phenotypically normal alfalfa genotypes (Medicago sativa L.) has demonstrated the existence of a distinct ctDNA genotype from each source. These unique restriction fragment patterns were utilized to identify maternal or paternal origin of ctDNA in hybrid plants from crosses involving the normal alfalfa genotypes as females and the yellow-green chlorophyll deficient sectors as males. Progeny from these crosses expressing the yellow-green sectored phenotypes contained paternal ctDNA in the chlorophyll deficient sectors and maternal ctDNA in the normal sectors, confirming biparental plastid inheritance. The existence of mixed cells containing both mutant and normal plastids at various stages of sorting-out was observed by transmission electron microscopy of mesophyll cells in mosaic tissue from hybrid plants. This observation verified the biparental transmission of plastids in alfalfa.

Original languageEnglish (US)
Pages (from-to)545-549
Number of pages5
JournalTheoretical and Applied Genetics
Volume76
Issue number4
DOIs
StatePublished - Oct 1 1988

Keywords

  • Alfalfa
  • Biparental plastid inheritance
  • Chlorophyll deficient mutants
  • ctDNA

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Fingerprint Dive into the research topics of 'Biparental inheritance of chloroplast DNA and the existence of heteroplasmic cells in alfalfa'. Together they form a unique fingerprint.

  • Cite this