Blood and bronchoalveolar lavage endothelin-1 levels in nocturnal asthma

Monica Kraft, William R. Beam, Sally E. Wenzel, Martin R. Zamora, Richard F. O'Brien, Richard J. Martin

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Endothelin-1 (ET-1) is a potent endogenous vasoconstrictor that was recently shown to exhibit bronchoconstrictive properties in vitro. In asthmatic patients, the expression of ET-1 peptide was also shown to be increased in endobronchial biopsies. Thus, we postulated that ET-1 could be higher in subjects with nocturnal asthma. Bronchoalveolar lavage (BAL) fluid levels of ET-1 were measured in 10 subjects with nocturnal asthma and 6 normal volunteers at 0400 h (nighttime groups) and in an additional 7 subjects with nocturnal asthma and 6 control subjects at 1600 h (daytime groups). ET-1 levels were significantly lower in the nighttime asthmatic group (median 39.3 pg/ml protein, 7.3 to 44.0 IQ) than in the nighttime control group (median 77.4 pg/mg protein, 46.8 to 121.9 IQ; p = 0.01) or the daytime asthmatic group (median 56.8 pg/mg protein, 51.6 to 97.4 IQ; p = 0.04). ET-1 levels did not differ significantly between the daytime asthmatic and the daytime control groups or between control groups. Additionally, the overnight reduction in lung function was significantly correlated with lower BAL ET-1 levels (r = 0.57, p = 0.05). These results suggest that ET-1 plays a role in the overnight worsening of asthma and perhaps is more tightly tissue bound, resulting in lower levels of BAL fluid.

Original languageEnglish (US)
Pages (from-to)947-952
Number of pages6
JournalAmerican journal of respiratory and critical care medicine
Volume149
Issue number4
StatePublished - Apr 1 1994
Externally publishedYes

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Bronchoalveolar Lavage
Endothelin-1
Asthma
Bronchoalveolar Lavage Fluid
Control Groups
Proteins
Vasoconstrictor Agents
Healthy Volunteers
Biopsy
Lung
Peptides

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Kraft, M., Beam, W. R., Wenzel, S. E., Zamora, M. R., O'Brien, R. F., & Martin, R. J. (1994). Blood and bronchoalveolar lavage endothelin-1 levels in nocturnal asthma. American journal of respiratory and critical care medicine, 149(4), 947-952.

Blood and bronchoalveolar lavage endothelin-1 levels in nocturnal asthma. / Kraft, Monica; Beam, William R.; Wenzel, Sally E.; Zamora, Martin R.; O'Brien, Richard F.; Martin, Richard J.

In: American journal of respiratory and critical care medicine, Vol. 149, No. 4, 01.04.1994, p. 947-952.

Research output: Contribution to journalArticle

Kraft, M, Beam, WR, Wenzel, SE, Zamora, MR, O'Brien, RF & Martin, RJ 1994, 'Blood and bronchoalveolar lavage endothelin-1 levels in nocturnal asthma', American journal of respiratory and critical care medicine, vol. 149, no. 4, pp. 947-952.
Kraft, Monica ; Beam, William R. ; Wenzel, Sally E. ; Zamora, Martin R. ; O'Brien, Richard F. ; Martin, Richard J. / Blood and bronchoalveolar lavage endothelin-1 levels in nocturnal asthma. In: American journal of respiratory and critical care medicine. 1994 ; Vol. 149, No. 4. pp. 947-952.
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