Blood oxyhemoglobin saturation measurements by blue-green spectral shift

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Previous work describing a resilient method for measuring oxyhemoglobin saturation using the blue-green spectral shift was performed using cell free hemoglobin solutions. Hemoglobin solution and whole blood sample spectra measured under similar conditions in a spectrophotometer are used here to begin evaluating the impact of cellular scattering on this method. The blue-green spectral shift with changing oxyhemoglobin saturation was preserved in these blood samples and the blue-green spectral shift was relatively unaffected by physiological changes in blood pH (6.6, 7.1, and 7.4), path length through blood (100 and 200 μm), and blood hematocrit (19 to 48%). The packaging of hemoglobin in red blood cells leads to a decreased apparent path length through hemoglobin, and an overall decrease in scattering loss with increasing wavelength from 450 to 850 nm. The negative slope of the scattering loss in the 476 to 516-nm range leads to a +3.0 nm shift in the oxyhemoglobin saturation calibration line when the blue-green spectral minimum in these blood samples was compared to cell free hemoglobin. Further research is needed to fully evaluate the blue green spectral shift method in cellular systems including in vivo testing.

Original languageEnglish (US)
Article number034020
JournalJournal of Biomedical Optics
Volume12
Issue number3
DOIs
StatePublished - May 2007

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oxyhemoglobin
Oxyhemoglobins
hemoglobin
blood
Hemoglobin
Blood
Hemoglobins
saturation
shift
Scattering
scattering
hematocrit
erythrocytes
Product Packaging
spectrophotometers
cells
Hematocrit
packaging
Spectrophotometers
Calibration

Keywords

  • Oximetry
  • Red blood cell scattering
  • Spectroscopy

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

Blood oxyhemoglobin saturation measurements by blue-green spectral shift. / Denninghoff, Kurt R; Chipman, Russell A; Hillman, Lloyd W.

In: Journal of Biomedical Optics, Vol. 12, No. 3, 034020, 05.2007.

Research output: Contribution to journalArticle

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