Bolalipid membrane structure revealed by solid-state 2H NMR spectroscopy

David P. Holland, Andrey V. Struts, Michael F Brown, David H. Thompson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Membranes made from three specifically deuterium-labeled ether-linked bolalipids, [1′,1′,20′,20′-2H4]C20BAS-PC, [2′,2′,19′,19′-2H4]C20BAS-PC, or [10′,11′-2H2]C20BAS-PC, were analyzed by 2H NMR spectroscopy. Unlike more common monopolar, ester-linked phospholipids, C20BAS-PC exhibits a high degree of orientational order throughout the membrane and the sn-1 chain of the lipid initially penetrates the bilayer at an orientation different from that of the bilayer normal, resulting in inequivalent deuterium atoms at the C1 position. The approximate hydrophobic layer thickness and area per lipid are 18.4 Å and 60.4 Å2, respectively, at 25 °C, and their respective thermal expansion coefficients are within 20% of the monopolar phospholipid, DLPC.

Original languageEnglish (US)
Pages (from-to)4584-4585
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number14
DOIs
StatePublished - Apr 9 2008

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Membrane structures
Deuterium
Phospholipids
Lipids
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Membranes
Ether
Thermal expansion
Ethers
Esters
Hot Temperature
Atoms

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Bolalipid membrane structure revealed by solid-state 2H NMR spectroscopy. / Holland, David P.; Struts, Andrey V.; Brown, Michael F; Thompson, David H.

In: Journal of the American Chemical Society, Vol. 130, No. 14, 09.04.2008, p. 4584-4585.

Research output: Contribution to journalArticle

Holland, David P. ; Struts, Andrey V. ; Brown, Michael F ; Thompson, David H. / Bolalipid membrane structure revealed by solid-state 2H NMR spectroscopy. In: Journal of the American Chemical Society. 2008 ; Vol. 130, No. 14. pp. 4584-4585.
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