Bone vitamin D-dependent calcium-binding protein is localized in chondrocytes of growth-plate cartilage

X. Y. Zhou, D. W. Dempster, S. L. Marion, J. W. Pike, Mark R Haussler, T. L. Clemens

Research output: Contribution to journalArticle

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Abstract

The cellular distribution of vitamin D-dependent calcium-binding protein (CaBP) was examined in rat and chicken bone by immunocytochemical methods using an antiserum raised against purified chicken intestinal CaBP. In EDTA-decalcified, Vibratome sections of growing rat long bones, specific CaBP immunostaining was observed in cytoplasm of chondrocytes of the growth plate, particularly in regions of calcification. In undecalcified, frozen sections from neonatal rat, positive staining was seen in chondrocytes of tibial growth plate and also in chondrocytes of the long bones of the skull. No specific immunostaining was observed in osteoblasts, osteocytes or osteoclasts in mineralized bone. In frozen sections of tibias from 19-day-old chick embryos specific immunostaining was again confined to dividing chondrocytes of the growth plate and was much less intense in "resting" cartilage. The finding of CaBP in chondrocytes, cells known to possess specific receptors for 1,25-dihydroxyvitamin D3 and to respond to the hormone, suggests a possible functional role for CaBP in chondrocyte maturation, differentiation and/or cartilage calcification.

Original languageEnglish (US)
Pages (from-to)244-247
Number of pages4
JournalCalcified Tissue International
Volume38
Issue number4
DOIs
StatePublished - Jul 1986

Fingerprint

S100 Calcium Binding Protein G
Growth Plate
Chondrocytes
Cartilage
Calcium-Binding Proteins
Bone and Bones
Frozen Sections
Chickens
Osteocytes
Calcitriol Receptors
Osteoclasts
Chick Embryo
Osteoblasts
Tibia
Skull
Edetic Acid
Immune Sera
Cytoplasm
Hormones
Staining and Labeling

Keywords

  • bone
  • cartilage
  • chondrocytes
  • immunocytochemistry
  • Vitamin D-dependent calcium-binding protein

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Bone vitamin D-dependent calcium-binding protein is localized in chondrocytes of growth-plate cartilage. / Zhou, X. Y.; Dempster, D. W.; Marion, S. L.; Pike, J. W.; Haussler, Mark R; Clemens, T. L.

In: Calcified Tissue International, Vol. 38, No. 4, 07.1986, p. 244-247.

Research output: Contribution to journalArticle

Zhou, X. Y. ; Dempster, D. W. ; Marion, S. L. ; Pike, J. W. ; Haussler, Mark R ; Clemens, T. L. / Bone vitamin D-dependent calcium-binding protein is localized in chondrocytes of growth-plate cartilage. In: Calcified Tissue International. 1986 ; Vol. 38, No. 4. pp. 244-247.
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