Bordetella bronchiseptica responses to physiological reactive nitrogen and oxygen stresses

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Bordetella bronchiseptica can establish prolonged airway infection consistent with a highly developed ability to evade mammalian host immune responses. Upon initial interaction with the host upper respiratory tract mucosa, B. bronchiseptica are subjected to antimicrobial reactive nitrogen species (RNS) and reactive oxygen species (ROS), effector molecules of the innate immune system. However, the responses of B. bronchiseptica to redox species at physiologically relevant concentrations (nM-μM) have not been investigated. Using predicted physiological concentrations of nitric oxide (NO), superoxide and hydrogen peroxide (H2O2) on low numbers of CFU of B. bronchiseptica, all redox active species displayed dose-dependent antimicrobial activity. Susceptibility to individual redox active species was significantly increased upon introduction of a second species at subantimicrobial concentrations. An increased bacteriostatic activity of NO was observed relative to H2O2. The understanding of Bordetella responses to physiologically relevant levels of exogenous RNS and ROS will aid in defining the role of endogenous production of these molecules in host innate immunity against Bordetella and other respiratory pathogens.

Original languageEnglish (US)
Pages (from-to)92-101
Number of pages10
JournalFEMS Microbiology Letters
Volume284
Issue number1
DOIs
StatePublished - Jul 1 2008

Keywords

  • Bacterial stress
  • Hydrogen peroxide
  • Nitric oxide
  • Reactive nitrogen species (RNS)
  • Reactive oxygen species (ROS)
  • Superoxide

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Bordetella bronchiseptica responses to physiological reactive nitrogen and oxygen stresses'. Together they form a unique fingerprint.

  • Cite this