Bordetella pertussis adenylate cyclase: Effects of affinity-purified adenylate cyclase on human polymorphonuclear leukocyte functions

Richard L Friedman, R. L. Fiederlein, L. Glasser, John N Galgiani

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Abstract

Affinity-purified adenylate cyclase (AC) of Bordetella pertussis, free of contaminating pertussis toxin, was demonstrted to have biological effects on human polymorphonuclear leukocytes (PMN). AC at doses of 25 and 50 μg/ml increased intracellular cAMP levels in the phagocytes 7.6- to 23.5-fold, respectively, above basal levels. AC inhibited PMN chemiluminescence, chemotaxis, and superoxide production in a dose-dependent manner. The 50% inhibitory dose for chemotaxis and chemiluminescence was 36.5 μg/ml; for superoxide generation it was 71.0 μg/ml. Although these PMN metabolic functions were impaired, no effect on phagocytic activity was observed.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalInfection and Immunity
Volume55
Issue number1
StatePublished - 1987

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Bordetella pertussis
Adenylyl Cyclases
Neutrophils
Luminescence
Superoxides
Leukocyte Chemotaxis
Pertussis Toxin
Chemotaxis
Phagocytes

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Bordetella pertussis adenylate cyclase: Effects of affinity-purified adenylate cyclase on human polymorphonuclear leukocyte functions",
abstract = "Affinity-purified adenylate cyclase (AC) of Bordetella pertussis, free of contaminating pertussis toxin, was demonstrted to have biological effects on human polymorphonuclear leukocytes (PMN). AC at doses of 25 and 50 μg/ml increased intracellular cAMP levels in the phagocytes 7.6- to 23.5-fold, respectively, above basal levels. AC inhibited PMN chemiluminescence, chemotaxis, and superoxide production in a dose-dependent manner. The 50{\%} inhibitory dose for chemotaxis and chemiluminescence was 36.5 μg/ml; for superoxide generation it was 71.0 μg/ml. Although these PMN metabolic functions were impaired, no effect on phagocytic activity was observed.",
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T2 - Effects of affinity-purified adenylate cyclase on human polymorphonuclear leukocyte functions

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AU - Fiederlein, R. L.

AU - Glasser, L.

AU - Galgiani, John N

PY - 1987

Y1 - 1987

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AB - Affinity-purified adenylate cyclase (AC) of Bordetella pertussis, free of contaminating pertussis toxin, was demonstrted to have biological effects on human polymorphonuclear leukocytes (PMN). AC at doses of 25 and 50 μg/ml increased intracellular cAMP levels in the phagocytes 7.6- to 23.5-fold, respectively, above basal levels. AC inhibited PMN chemiluminescence, chemotaxis, and superoxide production in a dose-dependent manner. The 50% inhibitory dose for chemotaxis and chemiluminescence was 36.5 μg/ml; for superoxide generation it was 71.0 μg/ml. Although these PMN metabolic functions were impaired, no effect on phagocytic activity was observed.

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