Affinity-purified adenylate cyclase (AC) of Bordetella pertussis, free of contaminating pertussis toxin, was demonstrted to have biological effects on human polymorphonuclear leukocytes (PMN). AC at doses of 25 and 50 μg/ml increased intracellular cAMP levels in the phagocytes 7.6- to 23.5-fold, respectively, above basal levels. AC inhibited PMN chemiluminescence, chemotaxis, and superoxide production in a dose-dependent manner. The 50% inhibitory dose for chemotaxis and chemiluminescence was 36.5 μg/ml; for superoxide generation it was 71.0 μg/ml. Although these PMN metabolic functions were impaired, no effect on phagocytic activity was observed.
ASJC Scopus subject areas
- Infectious Diseases