Bordetella pertussis filamentous hemagglutinin gene: molecular cloning of a potential coding sequence.

J. Reiser, R. L. Friedman, R. Germanier

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

We have constructed an expression library of Bordetella pertussis DNA sequences, cloned in Escherichia coli. Random DNA fragments were inserted into the beta-galactosidase gene of bacteriophage lambda gt11 and plaques were screened with antibodies directed against filamentous hemagglutinin. The antigen-positive clone obtained expressed B. pertussis sequences as polypeptides fused to beta-galactosidase. The fusion polypeptides reacted both with antibodies to beta-galactosidase and with antibodies to filamentous hemagglutinin. The positive clone contains B. pertussis DNA sequences as determined by specific hybridization to Bordetella DNA.

Original languageEnglish (US)
Pages (from-to)265-271
Number of pages7
JournalDevelopments in biological standardization
Volume61
StatePublished - Dec 1 1985

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Drug Discovery
  • Public Health, Environmental and Occupational Health

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