Bovine germinal vesicle oocyte and cumulus cell proteomics

E. Memili, D. Peddinti, L. A. Shack, B. Nanduri, Fiona M McCarthy, H. Sagirkaya, Shane C Burgess

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Germinal vesicle (GV) breakdown is fundamental for maturation of fully grown, developmentally competent, mammalian oocytes. Bidirectional communication between oocytes and surrounding cumulus cells (CC) is essential for maturation of a competent oocyte. However, neither the factors involved in this communication nor the mechanisms of their actions are well defined. Here, we define the proteomes of GV oocytes and their surrounding CC, including membrane proteins, using proteomics in a bovine model. We found that 4395 proteins were expressed in the CC and 1092 proteins were expressed in oocytes. Further, 858 proteins were common to both the CC and the oocytes. This first comprehensive proteome analysis of bovine oocytes and CC not only provides a foundation for signaling and cell physiology at the GV stage of oocyte development, but are also valuable for comparative studies of other stages of oocyte development at the molecular level. Furthermore, some of these proteins may represent molecular biomarkers for developmental potential of oocytes.

Original languageEnglish (US)
Pages (from-to)1107-1120
Number of pages14
JournalReproduction
Volume133
Issue number6
DOIs
StatePublished - Jun 2007
Externally publishedYes

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Cumulus Cells
Proteomics
Oocytes
Proteome
Proteins
Cell Physiological Phenomena
Membrane Proteins
Biomarkers

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Cell Biology
  • Endocrinology
  • Embryology

Cite this

Memili, E., Peddinti, D., Shack, L. A., Nanduri, B., McCarthy, F. M., Sagirkaya, H., & Burgess, S. C. (2007). Bovine germinal vesicle oocyte and cumulus cell proteomics. Reproduction, 133(6), 1107-1120. https://doi.org/10.1530/REP-06-0149

Bovine germinal vesicle oocyte and cumulus cell proteomics. / Memili, E.; Peddinti, D.; Shack, L. A.; Nanduri, B.; McCarthy, Fiona M; Sagirkaya, H.; Burgess, Shane C.

In: Reproduction, Vol. 133, No. 6, 06.2007, p. 1107-1120.

Research output: Contribution to journalArticle

Memili, E, Peddinti, D, Shack, LA, Nanduri, B, McCarthy, FM, Sagirkaya, H & Burgess, SC 2007, 'Bovine germinal vesicle oocyte and cumulus cell proteomics', Reproduction, vol. 133, no. 6, pp. 1107-1120. https://doi.org/10.1530/REP-06-0149
Memili E, Peddinti D, Shack LA, Nanduri B, McCarthy FM, Sagirkaya H et al. Bovine germinal vesicle oocyte and cumulus cell proteomics. Reproduction. 2007 Jun;133(6):1107-1120. https://doi.org/10.1530/REP-06-0149
Memili, E. ; Peddinti, D. ; Shack, L. A. ; Nanduri, B. ; McCarthy, Fiona M ; Sagirkaya, H. ; Burgess, Shane C. / Bovine germinal vesicle oocyte and cumulus cell proteomics. In: Reproduction. 2007 ; Vol. 133, No. 6. pp. 1107-1120.
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