Brassinosteroids regulate grain filling in rice

Chuan Yin Wu, Anthony Trieu, Parthiban Radhakrishnan, Shing F. Kwok, Sam Harris, Ke Zhang, Jiulin Wang, Jianmin Wan, Huqu Zhai, Suguru Takatsuto, Shogo Matsumoto, Shozo Fujiok, Kenneth A Feldmann, Roger I. Pennell

Research output: Contribution to journalArticle

199 Citations (Scopus)

Abstract

Genes controlling hormone levels have been used to increase grain yields in wheat (Triticum aestivum) and rice (Oryza sativa). We created transgenic rice plants expressing maize (Zea mays), rice, or Arabidopsis thaliana genes encoding sterol C-22 hydroxylases that control brassinosteroid (BR) hormone levels using a promoter that is active in only the stems, leaves, and roots. The transgenic plants produced more tillers and more seed than wild-type plants. The seed were heavier as well, especially the seed at the bases of the spikes that fill the least. These phenotypic changes brought about 15 to 44% increases in grain yield per plant relative to wild-type plants in greenhouse and field trials. Expression of the Arabidopsis C-22 hydroxylase in the embryos or endosperms themselves had no apparent effect on seed weight. These results suggested that BRs stimulate the flow of assimilate from the source to the sink. Microarray and photosynthesis analysis of transgenic plants revealed evidence of enhanced CO2 assimilation, enlarged glucose pools in the flag leaves, and increased assimilation of glucose to starch in the seed. These results further suggested that BRs stimulate the flow of assimilate. Plants have not been bred directly for seed filling traits, suggesting that genes that control seed filling could be used to further increase grain yield in crop plants.

Original languageEnglish (US)
Pages (from-to)2130-2145
Number of pages16
JournalPlant Cell
Volume20
Issue number8
DOIs
StatePublished - Aug 2008
Externally publishedYes

Fingerprint

Brassinosteroids
brassinosteroids
filling period
Seeds
rice
seeds
Genetically Modified Plants
grain yield
transgenic plants
assimilation (physiology)
Mixed Function Oxygenases
hormones
Arabidopsis
Triticum
Zea mays
glucose
genes
Hormones
tillers
Genes

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Wu, C. Y., Trieu, A., Radhakrishnan, P., Kwok, S. F., Harris, S., Zhang, K., ... Pennell, R. I. (2008). Brassinosteroids regulate grain filling in rice. Plant Cell, 20(8), 2130-2145. https://doi.org/10.1105/tpc.107.055087

Brassinosteroids regulate grain filling in rice. / Wu, Chuan Yin; Trieu, Anthony; Radhakrishnan, Parthiban; Kwok, Shing F.; Harris, Sam; Zhang, Ke; Wang, Jiulin; Wan, Jianmin; Zhai, Huqu; Takatsuto, Suguru; Matsumoto, Shogo; Fujiok, Shozo; Feldmann, Kenneth A; Pennell, Roger I.

In: Plant Cell, Vol. 20, No. 8, 08.2008, p. 2130-2145.

Research output: Contribution to journalArticle

Wu, CY, Trieu, A, Radhakrishnan, P, Kwok, SF, Harris, S, Zhang, K, Wang, J, Wan, J, Zhai, H, Takatsuto, S, Matsumoto, S, Fujiok, S, Feldmann, KA & Pennell, RI 2008, 'Brassinosteroids regulate grain filling in rice', Plant Cell, vol. 20, no. 8, pp. 2130-2145. https://doi.org/10.1105/tpc.107.055087
Wu CY, Trieu A, Radhakrishnan P, Kwok SF, Harris S, Zhang K et al. Brassinosteroids regulate grain filling in rice. Plant Cell. 2008 Aug;20(8):2130-2145. https://doi.org/10.1105/tpc.107.055087
Wu, Chuan Yin ; Trieu, Anthony ; Radhakrishnan, Parthiban ; Kwok, Shing F. ; Harris, Sam ; Zhang, Ke ; Wang, Jiulin ; Wan, Jianmin ; Zhai, Huqu ; Takatsuto, Suguru ; Matsumoto, Shogo ; Fujiok, Shozo ; Feldmann, Kenneth A ; Pennell, Roger I. / Brassinosteroids regulate grain filling in rice. In: Plant Cell. 2008 ; Vol. 20, No. 8. pp. 2130-2145.
@article{60355fcf677246f8a3c43d7127169527,
title = "Brassinosteroids regulate grain filling in rice",
abstract = "Genes controlling hormone levels have been used to increase grain yields in wheat (Triticum aestivum) and rice (Oryza sativa). We created transgenic rice plants expressing maize (Zea mays), rice, or Arabidopsis thaliana genes encoding sterol C-22 hydroxylases that control brassinosteroid (BR) hormone levels using a promoter that is active in only the stems, leaves, and roots. The transgenic plants produced more tillers and more seed than wild-type plants. The seed were heavier as well, especially the seed at the bases of the spikes that fill the least. These phenotypic changes brought about 15 to 44{\%} increases in grain yield per plant relative to wild-type plants in greenhouse and field trials. Expression of the Arabidopsis C-22 hydroxylase in the embryos or endosperms themselves had no apparent effect on seed weight. These results suggested that BRs stimulate the flow of assimilate from the source to the sink. Microarray and photosynthesis analysis of transgenic plants revealed evidence of enhanced CO2 assimilation, enlarged glucose pools in the flag leaves, and increased assimilation of glucose to starch in the seed. These results further suggested that BRs stimulate the flow of assimilate. Plants have not been bred directly for seed filling traits, suggesting that genes that control seed filling could be used to further increase grain yield in crop plants.",
author = "Wu, {Chuan Yin} and Anthony Trieu and Parthiban Radhakrishnan and Kwok, {Shing F.} and Sam Harris and Ke Zhang and Jiulin Wang and Jianmin Wan and Huqu Zhai and Suguru Takatsuto and Shogo Matsumoto and Shozo Fujiok and Feldmann, {Kenneth A} and Pennell, {Roger I.}",
year = "2008",
month = "8",
doi = "10.1105/tpc.107.055087",
language = "English (US)",
volume = "20",
pages = "2130--2145",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "8",

}

TY - JOUR

T1 - Brassinosteroids regulate grain filling in rice

AU - Wu, Chuan Yin

AU - Trieu, Anthony

AU - Radhakrishnan, Parthiban

AU - Kwok, Shing F.

AU - Harris, Sam

AU - Zhang, Ke

AU - Wang, Jiulin

AU - Wan, Jianmin

AU - Zhai, Huqu

AU - Takatsuto, Suguru

AU - Matsumoto, Shogo

AU - Fujiok, Shozo

AU - Feldmann, Kenneth A

AU - Pennell, Roger I.

PY - 2008/8

Y1 - 2008/8

N2 - Genes controlling hormone levels have been used to increase grain yields in wheat (Triticum aestivum) and rice (Oryza sativa). We created transgenic rice plants expressing maize (Zea mays), rice, or Arabidopsis thaliana genes encoding sterol C-22 hydroxylases that control brassinosteroid (BR) hormone levels using a promoter that is active in only the stems, leaves, and roots. The transgenic plants produced more tillers and more seed than wild-type plants. The seed were heavier as well, especially the seed at the bases of the spikes that fill the least. These phenotypic changes brought about 15 to 44% increases in grain yield per plant relative to wild-type plants in greenhouse and field trials. Expression of the Arabidopsis C-22 hydroxylase in the embryos or endosperms themselves had no apparent effect on seed weight. These results suggested that BRs stimulate the flow of assimilate from the source to the sink. Microarray and photosynthesis analysis of transgenic plants revealed evidence of enhanced CO2 assimilation, enlarged glucose pools in the flag leaves, and increased assimilation of glucose to starch in the seed. These results further suggested that BRs stimulate the flow of assimilate. Plants have not been bred directly for seed filling traits, suggesting that genes that control seed filling could be used to further increase grain yield in crop plants.

AB - Genes controlling hormone levels have been used to increase grain yields in wheat (Triticum aestivum) and rice (Oryza sativa). We created transgenic rice plants expressing maize (Zea mays), rice, or Arabidopsis thaliana genes encoding sterol C-22 hydroxylases that control brassinosteroid (BR) hormone levels using a promoter that is active in only the stems, leaves, and roots. The transgenic plants produced more tillers and more seed than wild-type plants. The seed were heavier as well, especially the seed at the bases of the spikes that fill the least. These phenotypic changes brought about 15 to 44% increases in grain yield per plant relative to wild-type plants in greenhouse and field trials. Expression of the Arabidopsis C-22 hydroxylase in the embryos or endosperms themselves had no apparent effect on seed weight. These results suggested that BRs stimulate the flow of assimilate from the source to the sink. Microarray and photosynthesis analysis of transgenic plants revealed evidence of enhanced CO2 assimilation, enlarged glucose pools in the flag leaves, and increased assimilation of glucose to starch in the seed. These results further suggested that BRs stimulate the flow of assimilate. Plants have not been bred directly for seed filling traits, suggesting that genes that control seed filling could be used to further increase grain yield in crop plants.

UR - http://www.scopus.com/inward/record.url?scp=57749108204&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57749108204&partnerID=8YFLogxK

U2 - 10.1105/tpc.107.055087

DO - 10.1105/tpc.107.055087

M3 - Article

C2 - 18708477

AN - SCOPUS:57749108204

VL - 20

SP - 2130

EP - 2145

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 8

ER -