BRB-seq: Ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

Daniel Alpern, Vincent Gardeux, Julie Russeil, Bastien Mangeat, Antonio C.A. Meireles-Filho, Romane Breysse, David Hacker, Bart Deplancke

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR.

Original languageEnglish (US)
Article number71
JournalGenome biology
Volume20
Issue number1
DOIs
StatePublished - Apr 19 2019
Externally publishedYes

Fingerprint

RNA Sequence Analysis
barcoding
transcriptomics
RNA
Gene Library
cDNA libraries
gene expression
transform
genome
tolerance
Genome
Gene Expression
Costs and Cost Analysis
gene
cost
Genes
genes
sampling
methodology
method

Keywords

  • Barcoding
  • Gene expression
  • qPCR
  • RNA-seq
  • Transcriptomics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

Cite this

Alpern, D., Gardeux, V., Russeil, J., Mangeat, B., Meireles-Filho, A. C. A., Breysse, R., ... Deplancke, B. (2019). BRB-seq: Ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing. Genome biology, 20(1), [71]. https://doi.org/10.1186/s13059-019-1671-x

BRB-seq : Ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing. / Alpern, Daniel; Gardeux, Vincent; Russeil, Julie; Mangeat, Bastien; Meireles-Filho, Antonio C.A.; Breysse, Romane; Hacker, David; Deplancke, Bart.

In: Genome biology, Vol. 20, No. 1, 71, 19.04.2019.

Research output: Contribution to journalArticle

Alpern, D, Gardeux, V, Russeil, J, Mangeat, B, Meireles-Filho, ACA, Breysse, R, Hacker, D & Deplancke, B 2019, 'BRB-seq: Ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing', Genome biology, vol. 20, no. 1, 71. https://doi.org/10.1186/s13059-019-1671-x
Alpern, Daniel ; Gardeux, Vincent ; Russeil, Julie ; Mangeat, Bastien ; Meireles-Filho, Antonio C.A. ; Breysse, Romane ; Hacker, David ; Deplancke, Bart. / BRB-seq : Ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing. In: Genome biology. 2019 ; Vol. 20, No. 1.
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