Abstract
Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR.
| Original language | English (US) |
|---|---|
| Article number | 71 |
| Journal | Genome biology |
| Volume | 20 |
| Issue number | 1 |
| DOIs | |
| State | Published - Apr 19 2019 |
| Externally published | Yes |
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Keywords
- Barcoding
- Gene expression
- qPCR
- RNA-seq
- Transcriptomics
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Genetics
- Cell Biology
Cite this
BRB-seq : Ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing. / Alpern, Daniel; Gardeux, Vincent; Russeil, Julie; Mangeat, Bastien; Meireles-Filho, Antonio C.A.; Breysse, Romane; Hacker, David; Deplancke, Bart.
In: Genome biology, Vol. 20, No. 1, 71, 19.04.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - BRB-seq
T2 - Ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing
AU - Alpern, Daniel
AU - Gardeux, Vincent
AU - Russeil, Julie
AU - Mangeat, Bastien
AU - Meireles-Filho, Antonio C.A.
AU - Breysse, Romane
AU - Hacker, David
AU - Deplancke, Bart
PY - 2019/4/19
Y1 - 2019/4/19
N2 - Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR.
AB - Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR.
KW - Barcoding
KW - Gene expression
KW - qPCR
KW - RNA-seq
KW - Transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=85064528030&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064528030&partnerID=8YFLogxK
U2 - 10.1186/s13059-019-1671-x
DO - 10.1186/s13059-019-1671-x
M3 - Article
C2 - 30999927
AN - SCOPUS:85064528030
VL - 20
JO - Genome Biology
JF - Genome Biology
SN - 1474-7596
IS - 1
M1 - 71
ER -