BRB-seq: Ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing

Daniel Alpern, Vincent Gardeux, Julie Russeil, Bastien Mangeat, Antonio C.A. Meireles-Filho, Romane Breysse, David Hacker, Bart Deplancke

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3′ cDNA libraries for dozens of samples, requiring just 2 hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR.

Original languageEnglish (US)
Article number71
JournalGenome biology
Volume20
Issue number1
DOIs
StatePublished - Apr 19 2019
Externally publishedYes

Keywords

  • Barcoding
  • Gene expression
  • qPCR
  • RNA-seq
  • Transcriptomics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Cell Biology

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    Alpern, D., Gardeux, V., Russeil, J., Mangeat, B., Meireles-Filho, A. C. A., Breysse, R., Hacker, D., & Deplancke, B. (2019). BRB-seq: Ultra-affordable high-throughput transcriptomics enabled by bulk RNA barcoding and sequencing. Genome biology, 20(1), [71]. https://doi.org/10.1186/s13059-019-1671-x