Breakthrough performance of linear-DNA on ion-exchange membrane columns

Rosa Ma Montesinos-Cisneros, Jaime Ortega, Roberto Z Guzman, Armando Tejeda-Mansir

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Breakthrough performance of linear-DNA adsorption on ion-exchange membrane columns was theoretically and experimentally investigated using batch and fixed-bed systems. System dispersion curves showed the absence of flow non-idealities in the experimental arrangement. Breakthrough curves were not significantly affected by flow-rate or inlet solution concentration. In the theoretical analysis a model was integrated by the serial coupling of the membrane transport model and the system dispersion model. A transport model that considers finite kinetic rate and column dispersed flow was used in the study. A simplex optimization routine coupled to the solution of the partial differential model equations was employed to estimate the maximum adsorption capacity constant, the equilibrium desorption constant and the forward interaction rate-constant, which are the parameters of the membrane transport model. Through this approach a good prediction of the adsorption phenomena is obtained for inlet concentrations and flow rates greater than 0.2 mg/ml and 0.16 ml/min.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalBioprocess and Biosystems Engineering
Volume29
Issue number2
DOIs
StatePublished - Jul 2006

Fingerprint

Ion exchange membranes
Ion Exchange
Adsorption
ion exchange
DNA
membrane
Membranes
adsorption
Flow rate
breakthrough curve
Rate constants
Desorption
desorption
kinetics
Kinetics
rate
prediction

Keywords

  • DNA
  • Frontal analysis
  • Ion-exchange
  • Membranes

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Bioengineering
  • Environmental Science (miscellaneous)

Cite this

Breakthrough performance of linear-DNA on ion-exchange membrane columns. / Montesinos-Cisneros, Rosa Ma; Ortega, Jaime; Guzman, Roberto Z; Tejeda-Mansir, Armando.

In: Bioprocess and Biosystems Engineering, Vol. 29, No. 2, 07.2006, p. 91-98.

Research output: Contribution to journalArticle

Montesinos-Cisneros, Rosa Ma ; Ortega, Jaime ; Guzman, Roberto Z ; Tejeda-Mansir, Armando. / Breakthrough performance of linear-DNA on ion-exchange membrane columns. In: Bioprocess and Biosystems Engineering. 2006 ; Vol. 29, No. 2. pp. 91-98.
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