Breakthrough performance of stacks of dye-cellulosic fabric in affinity chromatography of lysozyme

A. Tejeda-Mansir, R. M. Montesinos, I. Magaña-Plaza, R. Guzmán

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The breakthrough performance of stacks of dye-cellulosic fabric in affinity chromatography of lysozyme was investigated in batch and flow experiments. Breakthrough curves were significantly affected by flow rate and were not dependent on the feed solution concentration. System dispersion curves could not explain the flow-rate dependence. Breakthrough curves were analyzed by coupling the kinetic model for pore mass transfer as the only controlling resistance and a system dispersion model. From the analysis, pore film mass transfer resistance was found to be the leading rate-limiting factor when the residence time in the column is greater than 5 min. The model was used to predict the operating and design parameters needed to obtain sharp breakthrough curves. Selectivity studies using lysozyme and bovine serum albumin mixtures showed a high system selectivity for lysozyme.

Original languageEnglish (US)
Pages (from-to)235-242
Number of pages8
JournalBioprocess and Biosystems Engineering
Volume25
Issue number4
DOIs
StatePublished - Jan 2003

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Keywords

  • Affinity chromatography
  • Frontal analysis
  • Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

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