Breeding and characterization of soybean Triple Null; a stack of recessive alleles of Kunitz Trypsin Inhibitor, Soybean Agglutinin, and P34 allergen nulls

Monica Schmidt, Theodore Hymowitz, Eliot M Herman

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Soybean (Glycine max) seeds contain bioactive proteins with antinutritional and immunological properties that affect metabolism and assimilation of nutrients. The presence of antinutritional proteins requires soybeans to be heat-treated resulting in input energy costs. Nulls for bioactive seed proteins have been previously isolated from the USDA soybean collection, including Kunitz trypsin inhibitor (TI), soybean agglutinin (LE) and immunodominant soybean allergen P34 protein. Each of these nulls has the potential to partially address the concerns of soybean feed/food consumption. A stack of recessive nulls of TI, LE and P34 was created in a cv 'Williams 82' background termed 'Triple Null'. Triple Null has a slight reduction of total protein compared with 'Williams 82' corresponding to aggregate contribution of TI, LE and P34 in the seed proteome. Triple Null's proteome analysis revealed P34 and TI nulls are frame-shift mutants able to accumulate small amounts of authentic P34 and TI proteins. Triple Null has possible application as a conventional feed/food source and for immunotherapy to mitigate soybean allergenic response.

Original languageEnglish (US)
Pages (from-to)310-315
Number of pages6
JournalPlant Breeding
Volume134
Issue number3
DOIs
StatePublished - Jun 1 2015

Fingerprint

Trypsin Inhibitors
agglutinins
trypsin inhibitors
allergens
Soybeans
Allergens
Breeding
Alleles
soybeans
alleles
breeding
Seeds
Proteome
Food
proteome
Proteins
proteins
United States Department of Agriculture
seeds
Soybean Proteins

Keywords

  • Glycine max
  • Allergen
  • Kunitz trypsin inhibitor
  • P34
  • Seed lectin
  • Soybean
  • Soybean agglutinin

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Genetics

Cite this

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title = "Breeding and characterization of soybean Triple Null; a stack of recessive alleles of Kunitz Trypsin Inhibitor, Soybean Agglutinin, and P34 allergen nulls",
abstract = "Soybean (Glycine max) seeds contain bioactive proteins with antinutritional and immunological properties that affect metabolism and assimilation of nutrients. The presence of antinutritional proteins requires soybeans to be heat-treated resulting in input energy costs. Nulls for bioactive seed proteins have been previously isolated from the USDA soybean collection, including Kunitz trypsin inhibitor (TI), soybean agglutinin (LE) and immunodominant soybean allergen P34 protein. Each of these nulls has the potential to partially address the concerns of soybean feed/food consumption. A stack of recessive nulls of TI, LE and P34 was created in a cv 'Williams 82' background termed 'Triple Null'. Triple Null has a slight reduction of total protein compared with 'Williams 82' corresponding to aggregate contribution of TI, LE and P34 in the seed proteome. Triple Null's proteome analysis revealed P34 and TI nulls are frame-shift mutants able to accumulate small amounts of authentic P34 and TI proteins. Triple Null has possible application as a conventional feed/food source and for immunotherapy to mitigate soybean allergenic response.",
keywords = "Glycine max, Allergen, Kunitz trypsin inhibitor, P34, Seed lectin, Soybean, Soybean agglutinin",
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AU - Schmidt, Monica

AU - Hymowitz, Theodore

AU - Herman, Eliot M

PY - 2015/6/1

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N2 - Soybean (Glycine max) seeds contain bioactive proteins with antinutritional and immunological properties that affect metabolism and assimilation of nutrients. The presence of antinutritional proteins requires soybeans to be heat-treated resulting in input energy costs. Nulls for bioactive seed proteins have been previously isolated from the USDA soybean collection, including Kunitz trypsin inhibitor (TI), soybean agglutinin (LE) and immunodominant soybean allergen P34 protein. Each of these nulls has the potential to partially address the concerns of soybean feed/food consumption. A stack of recessive nulls of TI, LE and P34 was created in a cv 'Williams 82' background termed 'Triple Null'. Triple Null has a slight reduction of total protein compared with 'Williams 82' corresponding to aggregate contribution of TI, LE and P34 in the seed proteome. Triple Null's proteome analysis revealed P34 and TI nulls are frame-shift mutants able to accumulate small amounts of authentic P34 and TI proteins. Triple Null has possible application as a conventional feed/food source and for immunotherapy to mitigate soybean allergenic response.

AB - Soybean (Glycine max) seeds contain bioactive proteins with antinutritional and immunological properties that affect metabolism and assimilation of nutrients. The presence of antinutritional proteins requires soybeans to be heat-treated resulting in input energy costs. Nulls for bioactive seed proteins have been previously isolated from the USDA soybean collection, including Kunitz trypsin inhibitor (TI), soybean agglutinin (LE) and immunodominant soybean allergen P34 protein. Each of these nulls has the potential to partially address the concerns of soybean feed/food consumption. A stack of recessive nulls of TI, LE and P34 was created in a cv 'Williams 82' background termed 'Triple Null'. Triple Null has a slight reduction of total protein compared with 'Williams 82' corresponding to aggregate contribution of TI, LE and P34 in the seed proteome. Triple Null's proteome analysis revealed P34 and TI nulls are frame-shift mutants able to accumulate small amounts of authentic P34 and TI proteins. Triple Null has possible application as a conventional feed/food source and for immunotherapy to mitigate soybean allergenic response.

KW - Glycine max

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KW - Seed lectin

KW - Soybean

KW - Soybean agglutinin

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