Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide

Sergio B. Mendes, Lifeng Li, James J. Burke, John E. Lee, Darren R. Dunphy, S. Scott Saavedra

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

The development of a multichannel spectrometer based on single mode planar integrated optical waveguide and capable of measuring broad-band visible absorbance spectra of a weakly absorbing molecular film at a solid-liquid interface is reported. The system was successfully tested by measuring spectra, over a 100 nm bandwidth, of a hydrated film of cytochrome c adsorbed to a glass waveguide surface. The approach should prove useful in spectral characterization of structure and function in thin film molecular assemblies at condensed phase interfaces.

Original languageEnglish (US)
Pages (from-to)3374-3376
Number of pages3
JournalLangmuir
Volume12
Issue number14
StatePublished - Jul 10 1996

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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