Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide

Sergio B. Mendes, Lifeng Li, James J. Burke, John E. Lee, Darren R. Dunphy, Steven S Saavedra

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The development of a multichannel spectrometer based on single mode planar integrated optical waveguide and capable of measuring broad-band visible absorbance spectra of a weakly absorbing molecular film at a solid-liquid interface is reported. The system was successfully tested by measuring spectra, over a 100 nm bandwidth, of a hydrated film of cytochrome c adsorbed to a glass waveguide surface. The approach should prove useful in spectral characterization of structure and function in thin film molecular assemblies at condensed phase interfaces.

Original languageEnglish (US)
Pages (from-to)3374-3376
Number of pages3
JournalLangmuir
Volume12
Issue number14
StatePublished - Jul 10 1996

Fingerprint

Planar waveguides
Spectroscopy
broadband
waveguides
proteins
Proteins
Phase interfaces
cytochromes
Optical waveguides
liquid-solid interfaces
Cytochromes c
visible spectrum
optical waveguides
assemblies
spectroscopy
Spectrometers
Waveguides
spectrometers
bandwidth
Bandwidth

ASJC Scopus subject areas

  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry

Cite this

Mendes, S. B., Li, L., Burke, J. J., Lee, J. E., Dunphy, D. R., & Saavedra, S. S. (1996). Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide. Langmuir, 12(14), 3374-3376.

Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide. / Mendes, Sergio B.; Li, Lifeng; Burke, James J.; Lee, John E.; Dunphy, Darren R.; Saavedra, Steven S.

In: Langmuir, Vol. 12, No. 14, 10.07.1996, p. 3374-3376.

Research output: Contribution to journalArticle

Mendes, SB, Li, L, Burke, JJ, Lee, JE, Dunphy, DR & Saavedra, SS 1996, 'Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide', Langmuir, vol. 12, no. 14, pp. 3374-3376.
Mendes, Sergio B. ; Li, Lifeng ; Burke, James J. ; Lee, John E. ; Dunphy, Darren R. ; Saavedra, Steven S. / Broad-band attenuated total reflection spectroscopy of a hydrated protein film on a single mode planar waveguide. In: Langmuir. 1996 ; Vol. 12, No. 14. pp. 3374-3376.
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