BRS1, a serine carboxypeptidase, regulates BRI1 signaling in Arabidopsis thaliana

Jia Li, Kevin A. Lease, Frans Tax, John C. Walker

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

Brassinosteroid-insensitive 1 (BRI1) of Arabidopsis thaliana encodes a cell surface receptor for brassinosteroids. Mutations in BRI1 severely affect plant growth and development. Activation tagging of a weak bri1 allele (bri1-5) resulted in the identification of a new locus, brs1-1D. BRS1 is predicted to encode a secreted carboxypeptidase. Whereas a brs1 loss-of-function allele has no obvious mutant phenotype, overexpression of BRS1 can suppress bri1 extracellular domain mutants. Genetic analyses showed that brassinosteroids and a functional BRI1 protein kinase domain are required for suppression. In addition, overexpressed BRS1 missense mutants, predicted to abolish BRS1 protease activity, failed to suppress bri1-5. Finally, the effects of BRS1 are selective: over-expression in either wild-type or two other receptor kinase mutants resulted in no phenotypic alterations. These results strongly suggest that BRS1 processes a protein involved in an early event in the BRI1 signaling.

Original languageEnglish (US)
Pages (from-to)5916-5921
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number10
DOIs
StatePublished - May 8 2001
Externally publishedYes

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Brassinosteroids
Arabidopsis
Alleles
Carboxypeptidases
Plant Development
Cell Surface Receptors
Growth and Development
Protein Kinases
serine carboxypeptidase
Peptide Hydrolases
Phosphotransferases
Phenotype
Mutation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

BRS1, a serine carboxypeptidase, regulates BRI1 signaling in Arabidopsis thaliana. / Li, Jia; Lease, Kevin A.; Tax, Frans; Walker, John C.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 10, 08.05.2001, p. 5916-5921.

Research output: Contribution to journalArticle

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