BZU2/ZmMUTE controls symmetrical division of guard mother cell and specifies neighbor cell fate in maize

Hongliang Wang, Siyi Guo, Xin Qiao, Jianfei Guo, Zuliang Li, Yusen Zhou, Shenglong Bai, Zhiyong Gao, Daojie Wang, Pengcheng Wang, David W Galbraith, Chun Peng Song

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Intercellular communication in adjacent cell layers determines cell fate and polarity, thus orchestrating tissue specification and differentiation. Here we use the maize stomatal apparatus as a model to investigate cell fate determination. Mutations in ZmBZU2 (bizui2, bzu2) confer a complete absence of subsidiary cells (SCs) and normal guard cells (GCs), leading to failure of formation of mature stomatal complexes. Nuclear polarization and actin accumulation at the interface between subsidiary mother cells (SMCs) and guard mother cells (GMCs), an essential pre-requisite for asymmetric cell division, did not occur in Zmbzu2 mutants. ZmBZU2 encodes a basic helix-loop-helix (bHLH) transcription factor, which is an ortholog of AtMUTE in Arabidopsis (BZU2/ZmMUTE). We found that a number of genes implicated in stomatal development are transcriptionally regulated by BZU2/ZmMUTE. In particular, BZU2/ZmMUTE directly binds to the promoters of PAN1 and PAN2, two early regulators of protodermal cell fate and SMC polarization, consistent with the low levels of transcription of these genes observed in bzu2-1 mutants. BZU2/ZmMUTE has the cell-to-cell mobility characteristic similar to that of BdMUTE in Brachypodium distachyon. Unexpectedly, BZU2/ZmMUTE is expressed in GMC from the asymmetric division stage to the GMC division stage, and especially in the SMC establishment stage. Taken together, these data imply that BZU2/ZmMUTE is required for early events in SMC polarization and differentiation as well as for the last symmetrical division of GMCs to produce the two GCs, and is a master determinant of the cell fate of its neighbors through cell-to-cell communication.

Original languageEnglish (US)
Pages (from-to)e1008377
JournalPLoS genetics
Volume15
Issue number8
DOIs
StatePublished - Aug 1 2019

Fingerprint

Zea mays
Stem Cells
polarization
maize
corn
communication
gene
cells
mutation
Cell Division
cell communication
Brachypodium
Asymmetric Cell Division
guard cells
Basic Helix-Loop-Helix Transcription Factors
Cell Polarity
cell division
Arabidopsis
mutant
Cell Communication

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

BZU2/ZmMUTE controls symmetrical division of guard mother cell and specifies neighbor cell fate in maize. / Wang, Hongliang; Guo, Siyi; Qiao, Xin; Guo, Jianfei; Li, Zuliang; Zhou, Yusen; Bai, Shenglong; Gao, Zhiyong; Wang, Daojie; Wang, Pengcheng; Galbraith, David W; Song, Chun Peng.

In: PLoS genetics, Vol. 15, No. 8, 01.08.2019, p. e1008377.

Research output: Contribution to journalArticle

Wang, H, Guo, S, Qiao, X, Guo, J, Li, Z, Zhou, Y, Bai, S, Gao, Z, Wang, D, Wang, P, Galbraith, DW & Song, CP 2019, 'BZU2/ZmMUTE controls symmetrical division of guard mother cell and specifies neighbor cell fate in maize', PLoS genetics, vol. 15, no. 8, pp. e1008377. https://doi.org/10.1371/journal.pgen.1008377
Wang, Hongliang ; Guo, Siyi ; Qiao, Xin ; Guo, Jianfei ; Li, Zuliang ; Zhou, Yusen ; Bai, Shenglong ; Gao, Zhiyong ; Wang, Daojie ; Wang, Pengcheng ; Galbraith, David W ; Song, Chun Peng. / BZU2/ZmMUTE controls symmetrical division of guard mother cell and specifies neighbor cell fate in maize. In: PLoS genetics. 2019 ; Vol. 15, No. 8. pp. e1008377.
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AU - Guo, Siyi

AU - Qiao, Xin

AU - Guo, Jianfei

AU - Li, Zuliang

AU - Zhou, Yusen

AU - Bai, Shenglong

AU - Gao, Zhiyong

AU - Wang, Daojie

AU - Wang, Pengcheng

AU - Galbraith, David W

AU - Song, Chun Peng

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