C-C chemokines released by lipopolysaccharide (LPS)-stimulated human macrophages suppress HIV-1 infection in both macrophages and T cells

Alessia Verani, Gabriella Scarlatti, Manola Comar, Eleonora Tresoldi, Simona Polo, Mauro Giacca, Paolo Lusso, Antonio G. Siccardi, Donata Vercelli

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Human immunodeficiency virus-1 (HIV-1) expression in monocyte-derived macrophages (MDM) infected in vitro is known to be inhibited by lipopolysaccharide (LPS). However, the mechanisms are incompletely understood. We show here that HIV-1 suppression is mediated by soluble factors released by MDM stimulated with physiologically significant concentrations of LPS. LPS-conditioned supernatants from MDM inhibited HIV-1 replication in both MDM and T cells. Depletion of C-C chemokines (RANTES, MIP-1α, and MIP-1β) neutralized the ability of LPS-conditioned supernatants to inhibit HIV-1 replication in MDM. A combination of recombinant C-C chemokines blocked HIV-1 infection as effectively as LPS. Here, we report an inhibitory effect of C-C chemokines on HIV replication in primary macrophages. Our results raise the possibility that monocytes may play a dual role in HIV infection: while representing a reservoir for the virus, they may contribute to the containment of the infection by releasing factors that suppress HIV replication not only in monocytes but also in T lymphocytes.

Original languageEnglish (US)
Pages (from-to)805-816
Number of pages12
JournalJournal of Experimental Medicine
Volume185
Issue number5
DOIs
StatePublished - 1997
Externally publishedYes

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CC Chemokines
Virus Diseases
Lipopolysaccharides
HIV-1
Macrophages
T-Lymphocytes
Virus Replication
Monocytes
HIV
Chemokine CCL5
HIV Infections
Viruses
Infection

ASJC Scopus subject areas

  • Immunology

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C-C chemokines released by lipopolysaccharide (LPS)-stimulated human macrophages suppress HIV-1 infection in both macrophages and T cells. / Verani, Alessia; Scarlatti, Gabriella; Comar, Manola; Tresoldi, Eleonora; Polo, Simona; Giacca, Mauro; Lusso, Paolo; Siccardi, Antonio G.; Vercelli, Donata.

In: Journal of Experimental Medicine, Vol. 185, No. 5, 1997, p. 805-816.

Research output: Contribution to journalArticle

Verani, Alessia ; Scarlatti, Gabriella ; Comar, Manola ; Tresoldi, Eleonora ; Polo, Simona ; Giacca, Mauro ; Lusso, Paolo ; Siccardi, Antonio G. ; Vercelli, Donata. / C-C chemokines released by lipopolysaccharide (LPS)-stimulated human macrophages suppress HIV-1 infection in both macrophages and T cells. In: Journal of Experimental Medicine. 1997 ; Vol. 185, No. 5. pp. 805-816.
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AU - Tresoldi, Eleonora

AU - Polo, Simona

AU - Giacca, Mauro

AU - Lusso, Paolo

AU - Siccardi, Antonio G.

AU - Vercelli, Donata

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