C2 domain protein MIN1 promotes eyespot organization in Chlamydomonas reinhardtii

Telsa M. Mittelmeier, Peter Berthold, Avihai Danon, Mary Rose Lamb, Alexander Levitan, Michael E. Rice, Carol L Dieckmann

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Assembly and asymmetric localization of the photosensory eyespot in the biflagellate, unicellular green alga Chlamydomonas reinhardtii requires coordinated organization of photoreceptors in the plasma membrane and pigment granule/thylakoid membrane layers in the chloroplast. min1 (mini-eyed) mutant cells contain abnormally small, disorganized eyespots in which the chloroplast envelope and plasma membrane are no longer apposed. The MIN1 gene, identified here by phenotypic rescue, encodes a protein with an N-terminal C2 domain and a C-terminal LysM domain separated by a transmembrane sequence. This novel domain architecture led to the hypothesis that MIN1 is in the plasma membrane or the chloroplast envelope, where membrane association of the C2 domain promotes proper eyespot organization. Mutation of conserved C2 domain loop residues disrupted association of the MIN1 C2 domain with the chloroplast envelope in moss cells but did not abolish eyespot assembly in Chlamydomonas. In min1 null cells, channelrhodopsin-1 (ChR1) photoreceptor levels were reduced, indicating a role for MIN1 in ChR1 expression and/or stability. However, ChR1 localization was only minimally disturbed during photoautotrophic growth of min1 cells, conditions under which the pigment granule layers are disorganized. The data are consistent with the hypothesis that neither MIN1 nor proper organization of the plastidic components of the eyespot is essential for localization of ChR1.

Original languageEnglish (US)
Pages (from-to)2100-2112
Number of pages13
JournalEukaryotic Cell
Volume7
Issue number12
DOIs
StatePublished - Dec 2008

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Chlamydomonas reinhardtii
Chloroplasts
Cell Membrane
Proteins
Chlamydomonas
Bryophyta
Null Lymphocytes
Chlorophyta
Thylakoids
Mutation
Membranes
C2 Domains
Growth
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Mittelmeier, T. M., Berthold, P., Danon, A., Lamb, M. R., Levitan, A., Rice, M. E., & Dieckmann, C. L. (2008). C2 domain protein MIN1 promotes eyespot organization in Chlamydomonas reinhardtii. Eukaryotic Cell, 7(12), 2100-2112. https://doi.org/10.1128/EC.00118-08

C2 domain protein MIN1 promotes eyespot organization in Chlamydomonas reinhardtii. / Mittelmeier, Telsa M.; Berthold, Peter; Danon, Avihai; Lamb, Mary Rose; Levitan, Alexander; Rice, Michael E.; Dieckmann, Carol L.

In: Eukaryotic Cell, Vol. 7, No. 12, 12.2008, p. 2100-2112.

Research output: Contribution to journalArticle

Mittelmeier, TM, Berthold, P, Danon, A, Lamb, MR, Levitan, A, Rice, ME & Dieckmann, CL 2008, 'C2 domain protein MIN1 promotes eyespot organization in Chlamydomonas reinhardtii', Eukaryotic Cell, vol. 7, no. 12, pp. 2100-2112. https://doi.org/10.1128/EC.00118-08
Mittelmeier TM, Berthold P, Danon A, Lamb MR, Levitan A, Rice ME et al. C2 domain protein MIN1 promotes eyespot organization in Chlamydomonas reinhardtii. Eukaryotic Cell. 2008 Dec;7(12):2100-2112. https://doi.org/10.1128/EC.00118-08
Mittelmeier, Telsa M. ; Berthold, Peter ; Danon, Avihai ; Lamb, Mary Rose ; Levitan, Alexander ; Rice, Michael E. ; Dieckmann, Carol L. / C2 domain protein MIN1 promotes eyespot organization in Chlamydomonas reinhardtii. In: Eukaryotic Cell. 2008 ; Vol. 7, No. 12. pp. 2100-2112.
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