C7 M/N protein polymorphism typing applied to inherited deficiencies of human complement proteins C6 and C7

R. Wurzner, Naomi E Rance, P. C. Potter, M. L. Hendricks, P. J. Lachmann, A. Orren

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

C7 M/N typing, the determination of the complement component C7 M/N phenotypes, was successfully used in family studies to trace haplotypes bearing C7 deficiency genes. Furthermore, it was shown to be preferable to C7 allotyping based on isoelectric focusing (IEF) since it distinguishes two common alleles (C7*M and C7*N), whereas one common C7 IEF allele (C7*1) predominates in most populations. It is also the more sensitive method, as it enabled detection of very low amounts of abnormal C7 molecules in the third generation of a combined subtotal C6/C7-deficient subject and thus confirmed that this partial deficiency gene is not silent in heterozygotes. In this respect C7 M/N typing is even more informative than DNA restriction fragment length polymorphism typing which will assess the presence but not necessarily the functional status of a gene. C6 and C7 genes are tightly linked and therefore C7 M/N typing was also applied to tracing C6 deficiency genes in families. C6/C7 haplotype analysis of South African C6-deficient (C6Q0) subjects revealed a strong allelic association of C6*Q0 and C7*M.

Original languageEnglish (US)
Pages (from-to)485-489
Number of pages5
JournalClinical and Experimental Immunology
Volume89
Issue number3
StatePublished - 1992
Externally publishedYes

Fingerprint

Complement C7
Complement C6
Complement System Proteins
Genes
Isoelectric Focusing
Proteins
Haplotypes
Alleles
Myeloma Proteins
Heterozygote
Restriction Fragment Length Polymorphisms
Phenotype
DNA
Population

Keywords

  • C6 C7 deficiency
  • complement
  • linkage disequilibrium
  • meningococcal infection
  • polymorphism

ASJC Scopus subject areas

  • Immunology

Cite this

C7 M/N protein polymorphism typing applied to inherited deficiencies of human complement proteins C6 and C7. / Wurzner, R.; Rance, Naomi E; Potter, P. C.; Hendricks, M. L.; Lachmann, P. J.; Orren, A.

In: Clinical and Experimental Immunology, Vol. 89, No. 3, 1992, p. 485-489.

Research output: Contribution to journalArticle

Wurzner, R, Rance, NE, Potter, PC, Hendricks, ML, Lachmann, PJ & Orren, A 1992, 'C7 M/N protein polymorphism typing applied to inherited deficiencies of human complement proteins C6 and C7', Clinical and Experimental Immunology, vol. 89, no. 3, pp. 485-489.
Wurzner, R. ; Rance, Naomi E ; Potter, P. C. ; Hendricks, M. L. ; Lachmann, P. J. ; Orren, A. / C7 M/N protein polymorphism typing applied to inherited deficiencies of human complement proteins C6 and C7. In: Clinical and Experimental Immunology. 1992 ; Vol. 89, No. 3. pp. 485-489.
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