Ca-ATPase activity in the rabbit and bovine lens

D. Borchman, N. A. Delamere, C. A. Paterson

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Membrane-rich vesicle preparations of rabbit and bovine lenses were prepared in such a manner as to preserve ATPase activity. The lipid:protein ratio of these preparations was increased 22- to 33-fold with a 94% recovery of total phospholipid. Using this preparation, calcium stimulated ATPase was routinely determined in both individual lenses and in pooled specimens. The pattern of stimulation of ATPase activity by a range of calcium concentrations was found to be similar in membrane preparations of epithelium and cortex, from rabbit and bovine lenses. The concentration of calcium necessary for half-maximal stimulation of ATPase activity was approximately 10-6 M. Calcium concentrations in excess of 10-4 M reduced the ATPase activity. Calcium-ATPase was undetectable in the lens nuclear region of both species. The regional distribution of sodium-potassium ATPase was also measured.

Original languageEnglish (US)
Pages (from-to)982-987
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume29
Issue number6
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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