Caenorhabditis elegans SAND-1 is essential for RAB-7 function in endosomal traffic

Dmitry Poteryaev, Hanna Fares, Bruce Bowerman, Anne Spang

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

The small rab-GTPase RAB-7 acts in endosome and endosome to lysosome traffic. We identified SAND-1 as a protein required for RAB-7 function based on similarities between SAND-1 and RAB-7 RNAi phenotypes. Although the initial uptake of yolk protein in oocytes, or of soluble secreted (ss) GFP in coelomocytes, appeared normal, further transport along the endocytic traffic route was delayed in the absence of SAND-1 function, and yolk proteins failed to reach yolk granules efficiently. Moreover, in coelomocytes, ssGFP and BSA-Texas-Red were endocytosed but not transported to lysosomes. We show that SAND-1 is essential for RAB-7 function at the transition from early to late endosomes, but not for RAB-7 function at lysosomes.

Original languageEnglish (US)
Pages (from-to)301-312
Number of pages12
JournalEMBO Journal
Volume26
Issue number2
DOIs
StatePublished - Jan 24 2007

Keywords

  • C. elegans
  • Early to late endosome transport
  • Endosomes
  • RAB GTPases
  • RAB7

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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