Calcium Wave Propagation in Networks of Endothelial Cells: Model-based Theoretical and Experimental Study

Juexuan Long, Michael Junkin, Pak Kin Wong, James Hoying, Pierre Deymier

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

In this paper, we present a combined theoretical and experimental study of the propagation of calcium signals in multicellular structures composed of human endothelial cells. We consider multicellular structures composed of a single chain of cells as well as a chain of cells with a side branch, namely a "T" structure. In the experiments, we investigate the result of applying mechano-stimulation to induce signaling in the form of calcium waves along the chain and the effect of single and dual stimulation of the multicellular structure. The experimental results provide evidence of an effect of architecture on the propagation of calcium waves. Simulations based on a model of calcium-induced calcium release and cell-to-cell diffusion through gap junctions shows that the propagation of calcium waves is dependent upon the competition between intracellular calcium regulation and architecture-dependent intercellular diffusion.

Original languageEnglish (US)
Article numbere1002847
JournalPLoS computational biology
Volume8
Issue number12
DOIs
StatePublished - Dec 2012

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Modeling and Simulation
  • Ecology
  • Molecular Biology
  • Genetics
  • Cellular and Molecular Neuroscience
  • Computational Theory and Mathematics

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