Calculated ionisation potentials determine the oxidation of vanillin precursors by lignin peroxidase

Rimko Ten Have, Ivonne M.C.M. Rietjens, Sybe Hartmans, Henk J. Swarts, Jim A. Field

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

In view of the biocatalytic production of vanillin, this research focused on the lignin peroxidase (LiP) catalysed oxidation of naturally occurring phenolic derivatives: O-methyl ethers, O-acetyl esters, and O- glucosyl ethers. The ionisation potential (IP) of a series of model compounds was calculated and compared to their experimental conversion by LiP, defining a relative IP threshold of approximately 9.0 eV. Based on this threshold value only the O-acetyl esters and glucosides of isoeugenol and coniferyl alcohol would be potential LiP substrates. Both O-acetyl esters were tested and were shown to be converted to O-acetyl vanillin in molar yields of 51.8 and 2.3%, respectively.

Original languageEnglish (US)
Pages (from-to)390-392
Number of pages3
JournalFEBS Letters
Volume430
Issue number3
DOIs
StatePublished - Jul 3 1998

Keywords

  • Ionization potential
  • Lignin peroxidase
  • Vanillin

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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