Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics

Michael W Riggs, Deborah A. Schaefer

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Cryptosporidiosis, caused by the apicomplexan parasite Cryptosporidium parvum, is a moderate-to-severe diarrheal disease now recognized as one of the leading causes of morbidity and mortality in livestock globally, and in humans living in resource-limited parts of the world, particularly those with AIDS or malnourished individuals. This recognition has fueled efforts for the discovery of effective therapeutics. While recent progress in drug discovery has been encouraging, there are presently no acceptably effective parasite-specific drugs for the disease. The urgent need for new drug discovery or drug repurposing has also increased the need for refined animal models of clinical disease for therapeutic efficacy evaluation. Here, we describe an acute model of cryptosporidiosis using newborn calves to evaluate well-defined clinical and parasitological parameter outcomes, including the effect on diarrhea severity and duration, oocyst numbers produced, and multiple measures of clinical health. The model is highly reproducible and provides unequivocal direct measures of treatment efficacy on diarrhea severity and parasite replication.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages253-282
Number of pages30
DOIs
Publication statusPublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2052
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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Keywords

  • Bovine model
  • Clinical evaluation
  • Cryptosporidiosis
  • Cryptosporidium
  • Diarrhea quantitation
  • Oocyst quantitation
  • Therapeutic evaluation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Riggs, M. W., & Schaefer, D. A. (2020). Calf Clinical Model of Cryptosporidiosis for Efficacy Evaluation of Therapeutics. In Methods in Molecular Biology (pp. 253-282). (Methods in Molecular Biology; Vol. 2052). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9748-0_15