cAMP signaling regulates histone H3 phosphorylation and mitotic entry through a disruption of G2 progression

Pedro Rodriguez-Collazo, Sara K. Snyder, Rebecca C. Chiffer, Erin A. Bressler, Ty C. Voss, Eric P. Anderson, Hans Gottfried Genieser, Catharine Smith

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

cAMP signaling is known to have significant effects on cell growth, either inhibitory or stimulatory depending on the cell type. Study of cAMP-induced growth inhibition in mammalian somatic cells has focused mainly on the combined role of protein kinase A (PKA) and mitogen-activated protein (MAP) kinases in regulation of progression through the G1 phase of the cell cycle. Here we show that cAMP signaling regulates histone H3 phosphorylation in a cell cycle-dependent fashion, increasing it in quiescent cells but dramatically reducing it in cycling cells. The latter is due to a rapid and dramatic loss of mitotic histone H3 phosphorylation caused by a disruption in G2 progression, as evidenced by the inhibition of mitotic entry and decreased activity of the CyclinB/Cdk1 kinase. The inhibition of G2 progression induced through cAMP signaling is dependent on expression of the catalytic subunit of PKA and is highly sensitive to intracellular cAMP concentration. The mechanism by which G2 progression is inhibited is independent of both DNA damage and MAP kinase signaling. Our results suggest that cAMP signaling activates a G2 checkpoint by a unique mechanism and provide new insight into normal cellular regulation of G2 progression.

Original languageEnglish (US)
Pages (from-to)2855-2869
Number of pages15
JournalExperimental Cell Research
Volume314
Issue number15
DOIs
StatePublished - Sep 10 2008

Fingerprint

Histones
Phosphorylation
Mitogen-Activated Protein Kinases
Cell Cycle
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits
CDC2 Protein Kinase
G1 Phase
Growth
Cyclic AMP-Dependent Protein Kinases
DNA Damage

Keywords

  • cAMP signaling
  • Cell cycle
  • Cyclin B
  • Cyclin-dependent kinase
  • Histone phosphorylation
  • Protein kinase A

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rodriguez-Collazo, P., Snyder, S. K., Chiffer, R. C., Bressler, E. A., Voss, T. C., Anderson, E. P., ... Smith, C. (2008). cAMP signaling regulates histone H3 phosphorylation and mitotic entry through a disruption of G2 progression. Experimental Cell Research, 314(15), 2855-2869. https://doi.org/10.1016/j.yexcr.2008.06.022

cAMP signaling regulates histone H3 phosphorylation and mitotic entry through a disruption of G2 progression. / Rodriguez-Collazo, Pedro; Snyder, Sara K.; Chiffer, Rebecca C.; Bressler, Erin A.; Voss, Ty C.; Anderson, Eric P.; Genieser, Hans Gottfried; Smith, Catharine.

In: Experimental Cell Research, Vol. 314, No. 15, 10.09.2008, p. 2855-2869.

Research output: Contribution to journalArticle

Rodriguez-Collazo, P, Snyder, SK, Chiffer, RC, Bressler, EA, Voss, TC, Anderson, EP, Genieser, HG & Smith, C 2008, 'cAMP signaling regulates histone H3 phosphorylation and mitotic entry through a disruption of G2 progression', Experimental Cell Research, vol. 314, no. 15, pp. 2855-2869. https://doi.org/10.1016/j.yexcr.2008.06.022
Rodriguez-Collazo P, Snyder SK, Chiffer RC, Bressler EA, Voss TC, Anderson EP et al. cAMP signaling regulates histone H3 phosphorylation and mitotic entry through a disruption of G2 progression. Experimental Cell Research. 2008 Sep 10;314(15):2855-2869. https://doi.org/10.1016/j.yexcr.2008.06.022
Rodriguez-Collazo, Pedro ; Snyder, Sara K. ; Chiffer, Rebecca C. ; Bressler, Erin A. ; Voss, Ty C. ; Anderson, Eric P. ; Genieser, Hans Gottfried ; Smith, Catharine. / cAMP signaling regulates histone H3 phosphorylation and mitotic entry through a disruption of G2 progression. In: Experimental Cell Research. 2008 ; Vol. 314, No. 15. pp. 2855-2869.
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