Acute infections in beagle puppies with canine parainfluenza virus type 2 (CPI2), and CPI2 in combination with Bordetella bronchiseptica (Bb) produce bronchiolitis and increased airways responsiveness to aerosolized histamine during the acute infection. In order to determine whether these observations were associated with increased levels of eicosanoids, the stable metabolites of thromboxane A2 and prostacylin, thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) respectively, and leukotriene B4 were measured in the bronchoalveolar lavage (BAL) fluid of 25 beagle puppies (age = 76 ± 1 days, mean ± SEM) 3-4 days after no infection (control, n = 6), inoculation with both CPI2 and Bb (CPI2-Bb, n = 11), inoculation with CPI2 alone (CPI2, n = 4), and inoculation with Bb alone (Bb, n = 4). In addition, plasma levels of TXB2 and 6-keto-PGF1α were measured before and after infection in the CPI2-Bb and control groups. The BAL concentration of thromboxane B2 was increased in the CPI2-Bb group (520 ± 120 pg/ml), but not in the CPI2 (88 ± 40 pg/ml), Bb (235 ± 100 pg/ml), or control groups (120 ± 60 pg/ml, p < 0.01). There also was a borderline increase in BAL concentration of LTB4 in the CP12-Bb group. No differences were observed in the BAL concentration of 6-keto PGF1α. Furthermore, neither TXB2 nor PGF1α was elevated in the plasma of control or CPI2-Bb puppies. These data suggest that increased thromboxane concentrations in BAL fluid are associated with histamine hyperresponsiveness during acute infection in the CPI2-Bb group.
|Original language||English (US)|
|Number of pages||5|
|Journal||Prostaglandins, Leukotrienes and Essential Fatty Acids|
|State||Published - Nov 1991|
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology