Canine parainfluenza type 2 and Bordetella bronchiseptica infection produces increased bronchoalveolar lavage thromboxane concentrations in beagle puppies

Stuart F Quan, M. L. Witten, N. N. Dambro, R. J. Lemen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Acute infections in beagle puppies with canine parainfluenza virus type 2 (CPI2), and CPI2 in combination with Bordetella bronchiseptica (Bb) produce bronchiolitis and increased airways responsiveness to aerosolized histamine during the acute infection. In order to determine whether these observations were associated with increased levels of eicosanoids, the stable metabolites of thromboxane A2 and prostacylin, thromboxane B2 (TXB2) and 6-keto-prostaglandin F (6-keto-PGF) respectively, and leukotriene B4 were measured in the bronchoalveolar lavage (BAL) fluid of 25 beagle puppies (age = 76 ± 1 days, mean ± SEM) 3-4 days after no infection (control, n = 6), inoculation with both CPI2 and Bb (CPI2-Bb, n = 11), inoculation with CPI2 alone (CPI2, n = 4), and inoculation with Bb alone (Bb, n = 4). In addition, plasma levels of TXB2 and 6-keto-PGF were measured before and after infection in the CPI2-Bb and control groups. The BAL concentration of thromboxane B2 was increased in the CPI2-Bb group (520 ± 120 pg/ml), but not in the CPI2 (88 ± 40 pg/ml), Bb (235 ± 100 pg/ml), or control groups (120 ± 60 pg/ml, p < 0.01). There also was a borderline increase in BAL concentration of LTB4 in the CP12-Bb group. No differences were observed in the BAL concentration of 6-keto PGF. Furthermore, neither TXB2 nor PGF was elevated in the plasma of control or CPI2-Bb puppies. These data suggest that increased thromboxane concentrations in BAL fluid are associated with histamine hyperresponsiveness during acute infection in the CPI2-Bb group.

Original languageEnglish (US)
Pages (from-to)171-175
Number of pages5
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume44
Issue number3
DOIs
StatePublished - 1991

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Human parainfluenza virus 2
Bordetella Infections
Bordetella bronchiseptica
Paramyxoviridae Infections
Thromboxanes
Bronchoalveolar Lavage
Viruses
Canidae
Thromboxane B2
Leukotriene B4
Bronchoalveolar Lavage Fluid
Infection
Histamine
Plasmas
Control Groups
Bronchiolitis
Thromboxane A2
Fluids
Eicosanoids
Infection Control

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

@article{7f5f553e435e4f2782a62ad379988cbb,
title = "Canine parainfluenza type 2 and Bordetella bronchiseptica infection produces increased bronchoalveolar lavage thromboxane concentrations in beagle puppies",
abstract = "Acute infections in beagle puppies with canine parainfluenza virus type 2 (CPI2), and CPI2 in combination with Bordetella bronchiseptica (Bb) produce bronchiolitis and increased airways responsiveness to aerosolized histamine during the acute infection. In order to determine whether these observations were associated with increased levels of eicosanoids, the stable metabolites of thromboxane A2 and prostacylin, thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) respectively, and leukotriene B4 were measured in the bronchoalveolar lavage (BAL) fluid of 25 beagle puppies (age = 76 ± 1 days, mean ± SEM) 3-4 days after no infection (control, n = 6), inoculation with both CPI2 and Bb (CPI2-Bb, n = 11), inoculation with CPI2 alone (CPI2, n = 4), and inoculation with Bb alone (Bb, n = 4). In addition, plasma levels of TXB2 and 6-keto-PGF1α were measured before and after infection in the CPI2-Bb and control groups. The BAL concentration of thromboxane B2 was increased in the CPI2-Bb group (520 ± 120 pg/ml), but not in the CPI2 (88 ± 40 pg/ml), Bb (235 ± 100 pg/ml), or control groups (120 ± 60 pg/ml, p < 0.01). There also was a borderline increase in BAL concentration of LTB4 in the CP12-Bb group. No differences were observed in the BAL concentration of 6-keto PGF1α. Furthermore, neither TXB2 nor PGF1α was elevated in the plasma of control or CPI2-Bb puppies. These data suggest that increased thromboxane concentrations in BAL fluid are associated with histamine hyperresponsiveness during acute infection in the CPI2-Bb group.",
author = "Quan, {Stuart F} and Witten, {M. L.} and Dambro, {N. N.} and Lemen, {R. J.}",
year = "1991",
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T1 - Canine parainfluenza type 2 and Bordetella bronchiseptica infection produces increased bronchoalveolar lavage thromboxane concentrations in beagle puppies

AU - Quan, Stuart F

AU - Witten, M. L.

AU - Dambro, N. N.

AU - Lemen, R. J.

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N2 - Acute infections in beagle puppies with canine parainfluenza virus type 2 (CPI2), and CPI2 in combination with Bordetella bronchiseptica (Bb) produce bronchiolitis and increased airways responsiveness to aerosolized histamine during the acute infection. In order to determine whether these observations were associated with increased levels of eicosanoids, the stable metabolites of thromboxane A2 and prostacylin, thromboxane B2 (TXB2) and 6-keto-prostaglandin F1α (6-keto-PGF1α) respectively, and leukotriene B4 were measured in the bronchoalveolar lavage (BAL) fluid of 25 beagle puppies (age = 76 ± 1 days, mean ± SEM) 3-4 days after no infection (control, n = 6), inoculation with both CPI2 and Bb (CPI2-Bb, n = 11), inoculation with CPI2 alone (CPI2, n = 4), and inoculation with Bb alone (Bb, n = 4). In addition, plasma levels of TXB2 and 6-keto-PGF1α were measured before and after infection in the CPI2-Bb and control groups. The BAL concentration of thromboxane B2 was increased in the CPI2-Bb group (520 ± 120 pg/ml), but not in the CPI2 (88 ± 40 pg/ml), Bb (235 ± 100 pg/ml), or control groups (120 ± 60 pg/ml, p < 0.01). There also was a borderline increase in BAL concentration of LTB4 in the CP12-Bb group. No differences were observed in the BAL concentration of 6-keto PGF1α. Furthermore, neither TXB2 nor PGF1α was elevated in the plasma of control or CPI2-Bb puppies. These data suggest that increased thromboxane concentrations in BAL fluid are associated with histamine hyperresponsiveness during acute infection in the CPI2-Bb group.

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