Capillary electrophoresis with a UV light-emitting diode source for chemical monitoring of native and derivatized fluorescent compounds

Suminda Hapuarachchi, Gordon A. Janaway, Craig A Aspinwall

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

We report the utilization of a high power UV light-emitting diode for fluorescence detection (UV-LED-IF) in CE separations. CE-UV-LED-IF allows analysis of a range of environmentally and biologically important compounds, including PAHs and biogenic amines, including neurotransmitters, amino acids, proteins, and peptides, that have been derivatized with UV-excited fluorogenic labels, e.g., o-phthalic dicarbox-aldehyde/ β-mercaptoethanol (OPA/β-ME). The 365 nm UV-LED was used as a stable, low cost source for detection of UV-excited fluorescent compounds. UV-LED-IF was used with both zonal CE separations and MEKC. Native fluorescence detection of PAHs was accomplished with detection limits ranging from 10 nM to 1.3 μM. Detection limits for OPA/ β-ME-labeled glutamic acid and aspartic acid were 11 and 10 nM, respectively, for off-line labeling, and 47 and 47 nM, respectively, for on-line labeling, comparable to UV-laser-based systems. Analysis of OPA/ β-ME-labeled proteins and peptides was performed with 28 and 47 nM detection limits for BSA and myoglobin, respectively.

Original languageEnglish (US)
Pages (from-to)4052-4059
Number of pages8
JournalElectrophoresis
Volume27
Issue number20
DOIs
StatePublished - Oct 2006

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Capillary electrophoresis
Capillary Electrophoresis
Ultraviolet Rays
Ultraviolet radiation
Diodes
Fluorescence
Limit of Detection
Monitoring
Polycyclic aromatic hydrocarbons
Labeling
Amino Acids, Peptides, and Proteins
Micellar Electrokinetic Capillary Chromatography
Biogenic Amines
Mercaptoethanol
Myoglobin
Aspartic Acid
Aldehydes
Light emitting diodes
Neurotransmitter Agents
Labels

Keywords

  • Amino acids
  • Capillary electrophoresis
  • Chemical monitoring
  • Fluorescent detection
  • Light-emitting diode

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Capillary electrophoresis with a UV light-emitting diode source for chemical monitoring of native and derivatized fluorescent compounds. / Hapuarachchi, Suminda; Janaway, Gordon A.; Aspinwall, Craig A.

In: Electrophoresis, Vol. 27, No. 20, 10.2006, p. 4052-4059.

Research output: Contribution to journalArticle

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