Capillary gas chromatographic analysis of serum bile acids as the n-butyl ester-trimethylsilyl ether derivatives

Ashok K. Batta, Gerald Salen, Keshava R. Rapole, Manju Batta, David Earnest, David Alberts

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Gas chromatographic separations of n-butyl ester-trimethylsilyl ether derivatives of several common bile acids were compared with those of the corresponding methyl ester-trimethylsilyl ether derivatives on a CP-Sil-5 CB capillary column. Both types of derivatives were similarly resolved from each other. However, the n-butyl ester-trimethylsilyl ether derivatives of the bile acids showed longer retention times than the corresponding methyl ester-trimethylsilyl ethers and unlike the methyl ester-trimethylsilyl ether derivatives, were completely resolved from and eluted later than the trimethylsilyl ethers of common plasma sterols including sitosterol. A simplified method of plasma work-up for quantitation of bile acids and application of the above method in quantification of plasma bile acids in humans is described.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Issue number2
StatePublished - Mar 20 1998



  • Bile acid
  • n-Butyl ester-trimethylsilyl ether

ASJC Scopus subject areas

  • Chemistry(all)

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