Capillary gas chromatographic analysis of serum bile acids as the n-butyl ester-trimethylsilyl ether derivatives

Ashok K. Batta, Gerald Salen, Keshava R. Rapole, Manju Batta, David Earnest, David S Alberts

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Gas chromatographic separations of n-butyl ester-trimethylsilyl ether derivatives of several common bile acids were compared with those of the corresponding methyl ester-trimethylsilyl ether derivatives on a CP-Sil-5 CB capillary column. Both types of derivatives were similarly resolved from each other. However, the n-butyl ester-trimethylsilyl ether derivatives of the bile acids showed longer retention times than the corresponding methyl ester-trimethylsilyl ethers and unlike the methyl ester-trimethylsilyl ether derivatives, were completely resolved from and eluted later than the trimethylsilyl ethers of common plasma sterols including sitosterol. A simplified method of plasma work-up for quantitation of bile acids and application of the above method in quantification of plasma bile acids in humans is described.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalJournal of Chromatography B: Biomedical Applications
Volume706
Issue number2
DOIs
StatePublished - Mar 20 1998

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Bile Acids and Salts
Gas chromatography
Ether
Esters
Derivatives
Plasmas
Methyl Ethers
Ethers
Sterols
Gases

Keywords

  • Bile acid
  • n-Butyl ester-trimethylsilyl ether

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Capillary gas chromatographic analysis of serum bile acids as the n-butyl ester-trimethylsilyl ether derivatives. / Batta, Ashok K.; Salen, Gerald; Rapole, Keshava R.; Batta, Manju; Earnest, David; Alberts, David S.

In: Journal of Chromatography B: Biomedical Applications, Vol. 706, No. 2, 20.03.1998, p. 337-341.

Research output: Contribution to journalArticle

Batta, Ashok K. ; Salen, Gerald ; Rapole, Keshava R. ; Batta, Manju ; Earnest, David ; Alberts, David S. / Capillary gas chromatographic analysis of serum bile acids as the n-butyl ester-trimethylsilyl ether derivatives. In: Journal of Chromatography B: Biomedical Applications. 1998 ; Vol. 706, No. 2. pp. 337-341.
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