Carbohydrate-based Clostridium difficile vaccines

Mario A. Monteiro, Zuchao Ma, Lisa Bertolo, Yuening Jiao, Luis Arroyo, Douglas Hodgins, Michael Mallozzi, Gayatri Vedantam, Martin Sagermann, John Sundsmo, Herbert Chow

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Clostridium difficile is responsible for thousands of deaths each year and a vaccine would be welcomed, especially one that would disrupt bacterial maintenance, colonization and persistence in carriers and convalescent patients. Structural explorations at the University of Guelph (ON, Canada) discovered that C. difficile may express three phosphorylated polysaccharides, named PSI, PSII and PSIII; this review captures our recent efforts to create vaccines based on these glycans, especially PSII, the common antigen that has precipitated immediate attention. The authors describe the design and immunogenicity of vaccines composed of raw polysaccharides and conjugates thereof. So far, it has been observed that anti-PSII antibodies can be raised in farm animals, mice and hamster models; humans and horses carry anti-PSII IgA and IgG antibodies from natural exposure to C. difficile, respectively; phosphate is an indispensable immunogenic epitope and vaccine-induced PSII antibodies recognize PSII on C. difficile outer surface.

Original languageEnglish (US)
Pages (from-to)421-431
Number of pages11
JournalExpert Review of Vaccines
Volume12
Issue number4
DOIs
Publication statusPublished - Apr 2013

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Keywords

  • antibiotic-associated diarrhea
  • Clostridium difficile
  • conjugate
  • polysaccharide
  • PSI
  • PSII
  • PSIII
  • vaccine

ASJC Scopus subject areas

  • Immunology
  • Drug Discovery
  • Pharmacology
  • Molecular Medicine

Cite this

Monteiro, M. A., Ma, Z., Bertolo, L., Jiao, Y., Arroyo, L., Hodgins, D., ... Chow, H. (2013). Carbohydrate-based Clostridium difficile vaccines. Expert Review of Vaccines, 12(4), 421-431. https://doi.org/10.1586/erv.13.9