Cardiac hypertrophy with preserved contractile function after selective deletion of GLUT4 from the heart

E. Dale Abel, Helen C. Kaulbach, Rong Tian, James C A Hopkins, John Duffy, Thomas C Doetschman, Timo Minnemann, Mary Ellen Boers, Ed Hadro, Corinna Oberste-Berghaus, William Quist, Bradford B. Lowell, Joanne S. Ingwall, Barbara B. Kahn

Research output: Contribution to journalArticle

230 Citations (Scopus)

Abstract

Glucose enters the heart via GLUT1 and GLUT4 glucose transporters. GLUT4-deficient mice develop striking cardiac hypertrophy and die prematurely. Whether their cardiac changes are caused primarily by GLUT4 deficiency in cardiomyocytes or by metabolic changes resulting from the absence of GLUT4 in skeletal muscle and adipose tissue is unclear. To determine the role of GLUT4 in the heart we used cre-loxP recombination to generate G4H(-/-) mice in which GLUT4 expression is abolished in the heart but is present in skeletal muscle and adipose tissue. Life span and serum concentrations of insulin, glucose, FFAs, lactate, and β-hydroxybutyrate were normal. Basal cardiac glucose transport and GLUT1 expression were both increased approximately 3-fold in G4H(-/-) mice, but insulin-stimulated glucose uptake was abolished. G4H(-/-) mice develop modest cardiac hypertrophy associated with increased myocyte size and induction of atrial natriuretic and brain natriuretic peptide gene expression in the ventricles. Myocardial fibrosis did not occur. Basal and isoproterenol-stimulated isovolumic contractile performance was preserved. Thus, selective ablation of GLUT4 in the heart initiates a series of events that results in compensated cardiac hypertrophy.

Original languageEnglish (US)
Pages (from-to)1703-1714
Number of pages12
JournalJournal of Clinical Investigation
Volume104
Issue number12
StatePublished - Dec 1999
Externally publishedYes

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Cardiomegaly
Glucose
Adipose Tissue
Skeletal Muscle
Insulin
Hydroxybutyrates
Muscles
Facilitative Glucose Transport Proteins
Brain Natriuretic Peptide
Isoproterenol
Cardiac Myocytes
Muscle Cells
Genetic Recombination
Lactic Acid
Fibrosis
Gene Expression
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Abel, E. D., Kaulbach, H. C., Tian, R., Hopkins, J. C. A., Duffy, J., Doetschman, T. C., ... Kahn, B. B. (1999). Cardiac hypertrophy with preserved contractile function after selective deletion of GLUT4 from the heart. Journal of Clinical Investigation, 104(12), 1703-1714.

Cardiac hypertrophy with preserved contractile function after selective deletion of GLUT4 from the heart. / Abel, E. Dale; Kaulbach, Helen C.; Tian, Rong; Hopkins, James C A; Duffy, John; Doetschman, Thomas C; Minnemann, Timo; Boers, Mary Ellen; Hadro, Ed; Oberste-Berghaus, Corinna; Quist, William; Lowell, Bradford B.; Ingwall, Joanne S.; Kahn, Barbara B.

In: Journal of Clinical Investigation, Vol. 104, No. 12, 12.1999, p. 1703-1714.

Research output: Contribution to journalArticle

Abel, ED, Kaulbach, HC, Tian, R, Hopkins, JCA, Duffy, J, Doetschman, TC, Minnemann, T, Boers, ME, Hadro, E, Oberste-Berghaus, C, Quist, W, Lowell, BB, Ingwall, JS & Kahn, BB 1999, 'Cardiac hypertrophy with preserved contractile function after selective deletion of GLUT4 from the heart', Journal of Clinical Investigation, vol. 104, no. 12, pp. 1703-1714.
Abel, E. Dale ; Kaulbach, Helen C. ; Tian, Rong ; Hopkins, James C A ; Duffy, John ; Doetschman, Thomas C ; Minnemann, Timo ; Boers, Mary Ellen ; Hadro, Ed ; Oberste-Berghaus, Corinna ; Quist, William ; Lowell, Bradford B. ; Ingwall, Joanne S. ; Kahn, Barbara B. / Cardiac hypertrophy with preserved contractile function after selective deletion of GLUT4 from the heart. In: Journal of Clinical Investigation. 1999 ; Vol. 104, No. 12. pp. 1703-1714.
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