Cardiac-specific inducible and conditional gene targeting in mice

Thomas C Doetschman, Mohamad Azhar

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Mouse genetic engineering has revolutionized our understanding of the molecular and genetic basis of heart development and disease. This technology involves conditional tissue-specific and temporal transgenic and gene targeting approaches, as well as introduction of polymorphisms into the mouse genome. These approaches are increasingly used to elucidate the genetic pathways underlying tissue homeostasis, physiology, and pathophysiology of adult heart. They have also led to the development of clinically relevant models of human cardiac diseases. Here, we review the technologies and their limitations in general and the cardiovascular research community in particular.

Original languageEnglish (US)
Pages (from-to)1498-1512
Number of pages15
JournalCirculation Research
Volume110
Issue number11
DOIs
Publication statusPublished - May 25 2012

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Keywords

  • conditional knockout mice
  • Cre recombinase
  • heart
  • inducible gene targeting
  • transgenic mice

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

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