Carotenoid supplementation reduces erythema in human skin after simulated solar radiation exposure

Jeongmin Lee, Shuguang Jiang, Norman Levine, Ronald R Watson

Research output: Contribution to journalArticle

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Abstract

Excessive exposure to solar radiation, especially ultraviolet A (UVA: 320-400 nm) and ultraviolet B (UVB: 290-320 nm) radiation, may induce UV- carcinogenesis and erythema in the skin. Although the protective effects of carotenoids against skin lesions are still unclear, β-carotene has been proposed as an oral sun protectant. The purpose of this study was to determine the magnitude of the protective effects of oral α- and β-carotene supplementation for 24 weeks on UVA- and UVB-induced erythema in humans. While being exposed to UVA and UVB radiation, 22 subjects (11 men and 11 women) were supplemented with natural carotenoids for 24 weeks. Each day for the first 8 weeks, subjects were given 30 mg of natural carotenoids containing 29.4 mg of β-carotene, 0.36 mg of α-carotene, and traces of other carotenoids in vegetable oil. The natural carotenoid dose was progressively raised by 30-mg increments, at every 8 weeks, from 30 mg to 90 mg. Small areas (1 cm2) of the skin were exposed to increasing doses of UV light (16-42 mJ/cm2) to determine the minimal erythema dose (MED). MED was defined as a uniform pink color with well-defined borders. MED readings were obtained by visual inspection 24 hr postirradiation. Blood samples taken during supplementation were used to determine α- and β-carotene serum levels and for a lipid peroxidation analysis. During natural carotenoid supplementation, the MED of solar simulator radiation increased significantly (P < 0.05). After 24 weeks of supplementation, serum β-carotene levels were increased from 0.22 μg/ml (95% CI;0.16-0.27) to 1.72 μg/ml (95% CI;1.61- 1.83). Similarly, α-carotene serum levels increased from 0.07 μg/ml (95% CI;0.048-0.092) to 0.36 μg/ml (95% CI; 0.32-0.40). Serum lipid peroxidation was significantly (P < 0.05) inhibited in a dose-dependent manner during natural carotenoid supplementation. The present data suggest that supplementation with natural carotenoids may partially protect human skin from UVA- and UVB-induced erythema, although the magnitude of the protective effect is modest.

Original languageEnglish (US)
Pages (from-to)170-174
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume223
Issue number2
StatePublished - Feb 2000

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Carotenoids
Erythema
Solar radiation
Skin
Radiation
Radiation Exposure
Serum
Lipid Peroxidation
Lipids
Plant Oils
Solar System
Ultraviolet Rays
Ultraviolet radiation
Sun
Reading
Carcinogenesis
Blood

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Carotenoid supplementation reduces erythema in human skin after simulated solar radiation exposure. / Lee, Jeongmin; Jiang, Shuguang; Levine, Norman; Watson, Ronald R.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 223, No. 2, 02.2000, p. 170-174.

Research output: Contribution to journalArticle

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abstract = "Excessive exposure to solar radiation, especially ultraviolet A (UVA: 320-400 nm) and ultraviolet B (UVB: 290-320 nm) radiation, may induce UV- carcinogenesis and erythema in the skin. Although the protective effects of carotenoids against skin lesions are still unclear, β-carotene has been proposed as an oral sun protectant. The purpose of this study was to determine the magnitude of the protective effects of oral α- and β-carotene supplementation for 24 weeks on UVA- and UVB-induced erythema in humans. While being exposed to UVA and UVB radiation, 22 subjects (11 men and 11 women) were supplemented with natural carotenoids for 24 weeks. Each day for the first 8 weeks, subjects were given 30 mg of natural carotenoids containing 29.4 mg of β-carotene, 0.36 mg of α-carotene, and traces of other carotenoids in vegetable oil. The natural carotenoid dose was progressively raised by 30-mg increments, at every 8 weeks, from 30 mg to 90 mg. Small areas (1 cm2) of the skin were exposed to increasing doses of UV light (16-42 mJ/cm2) to determine the minimal erythema dose (MED). MED was defined as a uniform pink color with well-defined borders. MED readings were obtained by visual inspection 24 hr postirradiation. Blood samples taken during supplementation were used to determine α- and β-carotene serum levels and for a lipid peroxidation analysis. During natural carotenoid supplementation, the MED of solar simulator radiation increased significantly (P < 0.05). After 24 weeks of supplementation, serum β-carotene levels were increased from 0.22 μg/ml (95{\%} CI;0.16-0.27) to 1.72 μg/ml (95{\%} CI;1.61- 1.83). Similarly, α-carotene serum levels increased from 0.07 μg/ml (95{\%} CI;0.048-0.092) to 0.36 μg/ml (95{\%} CI; 0.32-0.40). Serum lipid peroxidation was significantly (P < 0.05) inhibited in a dose-dependent manner during natural carotenoid supplementation. The present data suggest that supplementation with natural carotenoids may partially protect human skin from UVA- and UVB-induced erythema, although the magnitude of the protective effect is modest.",
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