Abstract
Shrimp (Penaeus monodon and P. vannamei) specimens were submitted to the University of Arizona's Aquaculture Pathology Laboratory (UAZAPL) and to the Texas Veterinary Medical Diagnostic Laboratory (TVMDL) in 2007 and 2008 from shrimp-rearing facilities in India and Indonesia for histological testing. These were found to present prominent golden to greenish-brown needle-and plate-like birefringent crystals within multifocal hemocytic granulomas in the antennal gland tubules and peritubular hemal sinuses. Their appearance was very similar to melamine-cyanuric acid-induced crystals previously described from cat and dog kidneys with melamine-associated renal failure (MARF). Significant chronic mortalities were reported from the affected P. vannamei farms in Indonesia, but were not observed in the affected P. monodon facility in India. Shrimp feed was suspected as the source of melamine due to the similarity of the shrimp antennal gland lesions to those present in MARF. 'Normal' and 'suspect' feed samples from the facilities in Indonesia and India were sent to regional laboratories for analysis. Melamine was detected in 2 of 4 feed samples from an affected Indonesian farm. Melamine was not detected in 'normal' feed from the Indian facility, but it was found in 2 'suspect' samples (Feeds A and B) at levels of 183.39 and 112.50 ppm, respectively. A bioassay of Feed A with P. vannamei at UAZAPL confirmed that the melamine-contaminated feed induced prominent granulomas in the antennal gland with the characteristic crystals within 10 d of the first feeding, experimentally confirming the direct relationship of melamine-adulterated feed to the unique pathology observed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 107-112 |
| Number of pages | 6 |
| Journal | Diseases of Aquatic Organisms |
| Volume | 86 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2009 |
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Keywords
- Bioassay
- Cyanuric acid
- Histology
- Melamine
- Penaeid shrimp
- Penaeus monodon
- Penaeus vannamei
ASJC Scopus subject areas
- Aquatic Science
- Ecology, Evolution, Behavior and Systematics
Cite this
Case reports of melamine-induced pathology in penaeid shrimp fed adulterated feeds. / Lightner, Donald V; Pantoja-Morales, Carlos R; Redman, Rita M.; Hasson, Kenneth W.; Menon, Jayagopal Pozoth.
In: Diseases of Aquatic Organisms, Vol. 86, No. 2, 2009, p. 107-112.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Case reports of melamine-induced pathology in penaeid shrimp fed adulterated feeds
AU - Lightner, Donald V
AU - Pantoja-Morales, Carlos R
AU - Redman, Rita M.
AU - Hasson, Kenneth W.
AU - Menon, Jayagopal Pozoth
PY - 2009
Y1 - 2009
N2 - Shrimp (Penaeus monodon and P. vannamei) specimens were submitted to the University of Arizona's Aquaculture Pathology Laboratory (UAZAPL) and to the Texas Veterinary Medical Diagnostic Laboratory (TVMDL) in 2007 and 2008 from shrimp-rearing facilities in India and Indonesia for histological testing. These were found to present prominent golden to greenish-brown needle-and plate-like birefringent crystals within multifocal hemocytic granulomas in the antennal gland tubules and peritubular hemal sinuses. Their appearance was very similar to melamine-cyanuric acid-induced crystals previously described from cat and dog kidneys with melamine-associated renal failure (MARF). Significant chronic mortalities were reported from the affected P. vannamei farms in Indonesia, but were not observed in the affected P. monodon facility in India. Shrimp feed was suspected as the source of melamine due to the similarity of the shrimp antennal gland lesions to those present in MARF. 'Normal' and 'suspect' feed samples from the facilities in Indonesia and India were sent to regional laboratories for analysis. Melamine was detected in 2 of 4 feed samples from an affected Indonesian farm. Melamine was not detected in 'normal' feed from the Indian facility, but it was found in 2 'suspect' samples (Feeds A and B) at levels of 183.39 and 112.50 ppm, respectively. A bioassay of Feed A with P. vannamei at UAZAPL confirmed that the melamine-contaminated feed induced prominent granulomas in the antennal gland with the characteristic crystals within 10 d of the first feeding, experimentally confirming the direct relationship of melamine-adulterated feed to the unique pathology observed.
AB - Shrimp (Penaeus monodon and P. vannamei) specimens were submitted to the University of Arizona's Aquaculture Pathology Laboratory (UAZAPL) and to the Texas Veterinary Medical Diagnostic Laboratory (TVMDL) in 2007 and 2008 from shrimp-rearing facilities in India and Indonesia for histological testing. These were found to present prominent golden to greenish-brown needle-and plate-like birefringent crystals within multifocal hemocytic granulomas in the antennal gland tubules and peritubular hemal sinuses. Their appearance was very similar to melamine-cyanuric acid-induced crystals previously described from cat and dog kidneys with melamine-associated renal failure (MARF). Significant chronic mortalities were reported from the affected P. vannamei farms in Indonesia, but were not observed in the affected P. monodon facility in India. Shrimp feed was suspected as the source of melamine due to the similarity of the shrimp antennal gland lesions to those present in MARF. 'Normal' and 'suspect' feed samples from the facilities in Indonesia and India were sent to regional laboratories for analysis. Melamine was detected in 2 of 4 feed samples from an affected Indonesian farm. Melamine was not detected in 'normal' feed from the Indian facility, but it was found in 2 'suspect' samples (Feeds A and B) at levels of 183.39 and 112.50 ppm, respectively. A bioassay of Feed A with P. vannamei at UAZAPL confirmed that the melamine-contaminated feed induced prominent granulomas in the antennal gland with the characteristic crystals within 10 d of the first feeding, experimentally confirming the direct relationship of melamine-adulterated feed to the unique pathology observed.
KW - Bioassay
KW - Cyanuric acid
KW - Histology
KW - Melamine
KW - Penaeid shrimp
KW - Penaeus monodon
KW - Penaeus vannamei
UR - http://www.scopus.com/inward/record.url?scp=70349581894&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349581894&partnerID=8YFLogxK
U2 - 10.3354/dao02111
DO - 10.3354/dao02111
M3 - Article
VL - 86
SP - 107
EP - 112
JO - Diseases of Aquatic Organisms
JF - Diseases of Aquatic Organisms
SN - 0177-5103
IS - 2
ER -