Ca2+-ATPase activity in the human Lens

Douglas Borchman, Christopher A. Patenon, Nicholas A Delamere

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A membrane-rich preparation of paired human lenses was prepared in such a manner as to preserve ATPase activity. The 1ipid:protein ratio of these preparations was increased 12-fold with an 85% recovery of total phospholipid. The pattern of stimulation of ATPase activity by a range of calcium concentrations was found to be similar in membrane preparations of epithelium and cortex. The concentration of calcium necessary for half-maximal simulations of ATPase activity was approximately 10-6 M. Ca2+-ATPase activity is undetectable in the lens nuclear region. A shift in the sensitivity of lens epithelial Ca2+-ATPase activity was observed with increasing age concomitant with a general increase in Ca2+-ATPase activity suggesting age related modifications of the membrane.

Original languageEnglish (US)
Pages (from-to)1049-1054
Number of pages6
JournalCurrent Eye Research
Volume8
Issue number10
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Calcium-Transporting ATPases
Human Activities
Lenses
Adenosine Triphosphatases
Membranes
Calcium
Phospholipids
Epithelium
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Ca2+-ATPase activity in the human Lens. / Borchman, Douglas; Patenon, Christopher A.; Delamere, Nicholas A.

In: Current Eye Research, Vol. 8, No. 10, 1989, p. 1049-1054.

Research output: Contribution to journalArticle

Borchman, Douglas ; Patenon, Christopher A. ; Delamere, Nicholas A. / Ca2+-ATPase activity in the human Lens. In: Current Eye Research. 1989 ; Vol. 8, No. 10. pp. 1049-1054.
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