Caveolin-1 mediated expression and secretion of Kallikrein 6 in colon cancer cells

Rebecca S. Henkhaus, Upal Kunal Basu Roy, Dora Cavallo-Medved, Bonnie F. Sloane, Eugene W. Gerner, Natalia A. Ignatenko

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Abstract

Kallikreins are secreted proteases that may play a functional role and/or serve as a serum biomarker for the presence or progression of certain types of cancers. Kallikrein 6 (KLK6) has been shown to be upregulated in several types of cancers, including colon. The aims of this study were to elucidate pathways that influence KLK6 gene expression and KLK6 protein secretion in the HCT116 human colon cancer cells. Our data indicate a central role for caveolin-1 (CAV-1), the main structural protein of caveolae, in both KLK6 gene expression and protein secretion. Sucrose gradient subcellular fractionation reveals that CAV-1 and KLK6 colocalize to lipid raft domains in the plasma membrane of HCT116 cells. Furthermore, we show that CAV-1, although it does not directly interact with the KLK6 molecule, enhances KLK6 secretion from the cells. Deactivation of CAV-1, through SRC-mediated phosphorylation, decreased KLK6 secretion. We also demonstrate that, in colon cancer cells, CAV-1 increased the amount of phosphorylated AKT in cells by inhibiting the activity of the AKT-negative regulators PP1 and PP2A. This study demonstrates that proteins such as CAV-1 and AKT, which are known to be altered in colon cancer, affect KLK6 expression and KLK6 secretion.

Original languageEnglish (US)
Pages (from-to)140-148
Number of pages9
JournalNeoplasia
Volume10
Issue number2
DOIs
StatePublished - Feb 2008

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ASJC Scopus subject areas

  • Cancer Research

Cite this

Henkhaus, R. S., Roy, U. K. B., Cavallo-Medved, D., Sloane, B. F., Gerner, E. W., & Ignatenko, N. A. (2008). Caveolin-1 mediated expression and secretion of Kallikrein 6 in colon cancer cells. Neoplasia, 10(2), 140-148. https://doi.org/10.1593/neo.07817