Cavia porcellus as a model for experimental infection by trypanosoma cruzi

Yagahira E. Castro-Sesquen, Robert H. Gilman, Verónica Yauri, Noelia Angulo, Manuela Verastegui, Daniel E. Velásquez, Charles R Sterling, Diana Martin, Caryn Bern

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The guinea pig (Cavia porcellus) is a natural reservoir for Trypanosoma cruzi but has seldom been used as an experimental infection model. We developed a guinea pig infection model for acute and chronic Chagas disease. Seventy-two guinea pigs were inoculated intradermally with 104 trypomastigotes of T. cruzi strain Y (experimental group); 18 guinea pigs were used as control group. Eight animals from the experimental group and two from the control group were sacrificed 5, 15, 20, 25, 40, 55, 115, 165, and 365 days after inoculation. During the acute phase (15 to 55 days), we observed parasitemia (with a peak on day 20) and positive IgM and IgG Western blots with anti-shed acute-phase antigen bands. The cardiac tissue showed vasculitis, necrosis (on days 40 to 55), moderate to severe inflammation, and abundant amastigote nests. Smaller numbers of amastigote nests were also present in kidney, brain, and other organs. In the early chronic phase (115 to 165 days), parasitemia disappeared and antiT. cruzi IgG antibodies were still detectable. In cardiac tissue, the number of amastigote nests and the grade of inflammation decreased. In the chronic phase (365 days), the cardiac tissue showed vasculitis and fibrosis; detectable parasite DNA was associated with higher grades of inflammation. The experimental T. cruzi infection model in guinea pigs shows kinetics and pathologic changes similar to those of the human disease.

Original languageEnglish (US)
Pages (from-to)281-288
Number of pages8
JournalAmerican Journal of Pathology
Volume179
Issue number1
DOIs
StatePublished - Jul 2011

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Trypanosoma cruzi
Guinea Pigs
Theoretical Models
Infection
Parasitemia
Vasculitis
Inflammation
Immunoglobulin G
Control Groups
Chagas Disease
Immunoglobulin M
Parasites
Fibrosis
Chronic Disease
Necrosis
Western Blotting
Kidney
Antigens
Antibodies
DNA

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Castro-Sesquen, Y. E., Gilman, R. H., Yauri, V., Angulo, N., Verastegui, M., Velásquez, D. E., ... Bern, C. (2011). Cavia porcellus as a model for experimental infection by trypanosoma cruzi. American Journal of Pathology, 179(1), 281-288. https://doi.org/10.1016/j.ajpath.2011.03.043

Cavia porcellus as a model for experimental infection by trypanosoma cruzi. / Castro-Sesquen, Yagahira E.; Gilman, Robert H.; Yauri, Verónica; Angulo, Noelia; Verastegui, Manuela; Velásquez, Daniel E.; Sterling, Charles R; Martin, Diana; Bern, Caryn.

In: American Journal of Pathology, Vol. 179, No. 1, 07.2011, p. 281-288.

Research output: Contribution to journalArticle

Castro-Sesquen, YE, Gilman, RH, Yauri, V, Angulo, N, Verastegui, M, Velásquez, DE, Sterling, CR, Martin, D & Bern, C 2011, 'Cavia porcellus as a model for experimental infection by trypanosoma cruzi', American Journal of Pathology, vol. 179, no. 1, pp. 281-288. https://doi.org/10.1016/j.ajpath.2011.03.043
Castro-Sesquen YE, Gilman RH, Yauri V, Angulo N, Verastegui M, Velásquez DE et al. Cavia porcellus as a model for experimental infection by trypanosoma cruzi. American Journal of Pathology. 2011 Jul;179(1):281-288. https://doi.org/10.1016/j.ajpath.2011.03.043
Castro-Sesquen, Yagahira E. ; Gilman, Robert H. ; Yauri, Verónica ; Angulo, Noelia ; Verastegui, Manuela ; Velásquez, Daniel E. ; Sterling, Charles R ; Martin, Diana ; Bern, Caryn. / Cavia porcellus as a model for experimental infection by trypanosoma cruzi. In: American Journal of Pathology. 2011 ; Vol. 179, No. 1. pp. 281-288.
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